Phenobarbital analysis in biological matrix (blood) by high performance liquid chromatography (HPLC)

• Autorzy: Hamza O. , Mohamed Moktar B. , Chohra Mostafa B. , Zeghdaoui A.
• Języki publikacji: EN

Abstrakty

EN

In this work, we have tried to contribute to the analysis of phenobarbital in the blood. To do this, we used different analytical techniques such as liquid chromatography (HPLC). The results obtained in the course of our work, we can conclude that: The HPLC method was separate phenobarbital endogenous blood components, and optimizing the conditions of chromatographic separation as the composition of the mobile phase consisting of 20 % acetonitrile + 20 % methanol + 60 % ammonium acetate buffer. The extraction with diethyl ether to pH = 4; The chromatographic column, microbondapack ZORBAX C18. A system of diode-array UV detection at 254 nm.

Słowa kluczowe

EN

Phenobarbital; HPLC; blood; extraction; biological matrix

• Wydawca: Przedsiębiorstwo Wydawnictw Naukowych "DARWIN"
• Czasopismo: International Letters of Chemistry, Physics and Astronomy
• Rocznik: 2014
• Tom: Vol. 1
• Strony: 31--40
• Opis fizyczny: Bibliogr. 8 poz., rys., wykr.

Twórcy

autor

Hamza O.

• Ecole Supérieure de Police-Chateauneuf, Laboratory of Scientific Police Cité El Ziania, ben Aknoun Algiers, Algeria

autor

Mohamed Moktar B.

• Ecole Supérieure de Police-Chateauneuf, Laboratory of Scientific Police Cité El Ziania, ben Aknoun Algiers, Algeria

autor

Chohra Mostafa B.

• Ecole Supérieure de Police-Chateauneuf, Laboratory of Scientific Police Cité El Ziania, ben Aknoun Algiers, Algeria

autor

Zeghdaoui A.

• Ecole Supérieure de Police-Chateauneuf, Laboratory of Scientific Police Cité El Ziania, ben Aknoun Algiers, Algeria

Bibliografia

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