Comparison between conventional solid phase extraction and its simplified method for HPLC determination of five flavonoids in orange, tangerine, and lime juice samples

• Autorzy: Asghari A. , Barfi B. , Barfi A. , Saeidi I. , Ghollasi-Moud F. , Peyrovi M. , Beig-Babaei A.
• Języki publikacji: EN

Abstrakty

EN

Although conventional solid phase extraction (SPE) is an applicable routine method for extraction of different analytes from various matrices, it requires more time and volume of solvent and sample rather than other routine laboratory extraction methods. In this study, a rapid and simplified sample preparation method based on SPE was studied by eliminating some steps, such as conditioning and washing, for extraction of diosmin, eriocitrin, narirutin, naringin, and hesperidin in orange, tangerine, and lime juice samples. The separation of these flavonoids was achieved by using a C8 column with a mobile phase comprised of water-acetonitrile-acetic acid (78:21:1, v/v/v) at a flow rate of 0.85 mL min^-1 and UV detection at 280 nm. To examine the applicability of this method, effective parameters such as type of adsorbent, type and volume of elution solvent, ionic strength, and pH of the sample were studied and applied to the comparison between the conventional SPE and the simplified method. The best recoveries were obtained, using the proposed method, with a small volume of citrus fruit juice (0.5 mL), silica gel (0.5 g) as adsorbent, and 3 mL of methanol as elution solvent. Limits of detection, limits of quantification, intra-day and inter-day precision of the method for the analytes were 0.0244–0.0587 μg mL^-1, 0.0739–0.178 μg mL^-1, 2.5 3.1%, and 3.1–4.8%, respectively.

Słowa kluczowe

EN

citrus fruit juice; flavonoid; HPLC; SPE

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2014
• Tom: Vol. 26, no. 1
• Strony: 157--175
• Opis fizyczny: Bibliogr. 18 poz., rys., tab.

Twórcy

autor

Asghari A.

• Semnan University Department of Chemistry Semnan 35195-363 Iran

autor

Barfi B.

• Semnan University Department of Chemistry Semnan 35195-363 Iran

autor

Barfi A.

• Arak University Department of Biology Arak Iran

autor

Saeidi I.

• Institute of Standard and Industrial Research of Iran Mashhad Iran

autor

Ghollasi-Moud F.

• Institute of Standard and Industrial Research of Iran Mashhad Iran

autor

Peyrovi M.

• Mazandran University Department of Chemistry Babolsar Iran

autor

Beig-Babaei A.

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