Cytotoxicity of Resorcinol Under Short- and Long-Term Exposure in Vitro

Skowroń, J.; Zapór, L.

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Tytuł artykułu

Cytotoxicity of Resorcinol Under Short- and Long-Term Exposure in Vitro

Autorzy

Skowroń J. , Zapór L.

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Abstrakty

Cytotoxicity of resorcinol to 3T3 fibroblast in short- (3 hrs) and long-term (72 hrs or 6 weeks) exposure was investigated. The effects of resorcinol on cell viability (neutral red uptake, NRU assay), mitochondrial function (MTT assay) and total cell protein (Kenacid Blue assay) were estimated. As a model for long-term exposure an INTEGRA CL 6-WELL bioreactor was used. The concentrations of resorcinol producing 20, 50 and 80% inhibition of cell growth in the NRU test were lower than in the MTT test after 3 hrs of exposure. The use of an INTEGRA CL 6-WELL bioreactor allows continuous culturing and exposure to test chemical of cells for several weeks, but the strong adhesiveness of fibroblast and forming aggregates make it difficult to remove them from chambers. Resorcinol in concentration of 1 μ/cm³ did not decrease the viability of cells to 50% of control in long-term exposure in the bioreactor.

Słowa kluczowe

resorcinol short-term toxicity long-term toxicity bioreactor in vitro methods

rezorcynol toksyczność chroniczna działanie toksyczne substancje toksyczne substancje niebezpieczne ekspozycja długotrwała ekspozycja chwilowa zatrucia

Wydawca

Taylor & Francis

Czasopismo

International Journal of Occupational Safety and Ergonomics

Rocznik

2004

Tom

Vol. 10, No. 2

Strony

147--156

Opis fizyczny

Bibliogr. 34 poz., rys., tab.

Twórcy

autor

Skowroń J.

josko@ciop.pl

Department of Chemical and Aerosol Hazards, Central Institute for Labour Protection – National Research Institute, Warsaw, Poland

autor

Zapór L.

Department of Chemical and Aerosol Hazards, Central Institute for Labour Protection – National Research Institute, Warsaw, Poland

Bibliografia

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Bibliografia

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