Development and validation of UPLC–MS/MS method for determination of eupatilin in rat plasma and its application in a pharmacokinetics study

• Autorzy: Geng P. , Luo X. , Peng X. , Lin Z. , Chen W. , Zhang J. , Wen C. , Hu L. , Hu S.

Identyfikatory

DOI

10.1556/1326.2017.00320

• Języki publikacji: EN

Abstrakty

EN

Eupatilin, mainly derived from Artemisia asiatica (Asteraceae), is an O-methylated flavone with various bioactivities. In the present study, a validated ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was established for the quantification of eupatilin in rat plasma with the internal standard (IS) of tussilagone and the protein precipitation of plasma samples was performed using acetonitrile–methanol (9:1, v/v). The eupatilin and IS were eluted separately on a UPLC BEH C18 column (2.1 mm × 100 mm, 1.7 μm) with the gradient mobile phase consisted of 0.1% formic acid and acetonitrile. The protonated analytes were quantified by multiple reactions monitoring (MRM) mode with an electrospray ionization (ESI) source operated in positive ion mode. The calibration plots were found to be linear over the range from 2 to 1000 ng/mL for eupatilin in rat plasma. Both of the intra-day and inter-day precision variations (RSDs) were ≤13%. The recoveries of eupatilin in rat plasma were between 83.7% and 94.6%, and the accuracy of the method ranged from 95.8% to 107.6%. In addition, the validated method was applied to pharmacokinetic study of eupatilin after an intravenous dose of 2 mg/kg to rats.

Słowa kluczowe

EN

Eupatilin; UPLC–MS/MS; rat plasma; pharmacokinetics

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2018
• Tom: Vol. 30, no. 4
• Strony: 231--235
• Opis fizyczny: Bibliogr. 30 poz., rys.

Twórcy

autor

Geng P.

• The Laboratory of Clinical Pharmacy, The People's Hospital of Lishui, Lishui 323000, China

autor

Luo X.

• Department of Clinical Lab Medicine, Taizhou Municipal Hospital affiliated with Taizhou University, Taizhou 318000, China

autor

Peng X.

• Laboratory Animal Centre, Wenzhou Medical University, Wenzhou 325035, China

autor

Lin Z.

• Laboratory Animal Centre, Wenzhou Medical University, Wenzhou 325035, China

autor

Chen W.

• Laboratory Animal Centre, Wenzhou Medical University, Wenzhou 325035, China

autor

Zhang J.

• The Laboratory of Clinical Pharmacy, The People's Hospital of Lishui, Lishui 323000, China

autor

Wen C.

• Laboratory Animal Centre, Wenzhou Medical University, Wenzhou 325035, China

autor

Hu L.

• The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China

autor

Hu S.

• The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China

Bibliografia

• 1. Nakasugi T.; Nakashima M.; Komai K. J. Agric. Food Chem. 2000, 48, 3256–3266.
• 2. Kim M. J.; Kim D. H.; Na H. K.; Oh T. Y.; Shin C. Y.; Surh PhD. P. Y. J. J Environ. Pathol. Toxicol. Oncol. 2005, 24, 261–269.
• 3. Kim D. H.; Na H. K.; Oh T. Y.; Kim W. B.; Surh Y. J. Biochem. Pharmacol. 2004 68, 1081–1087.
• 4. Seo H. J.; Surh Y. J. Mutat. Res. 2001, 496, 191–198.
• 5. Kang Y. J.; Jung U. J.; Lee M. K.; Kim H. J.; Jeon S. M.; Park Y. B.; Chung H. G.; Baek N. I.; Lee K. T.; Jeong T. S.; Choi M. S. Diabetes Res. Clin. Pract. 2008, 82, 25–32.
• 6. Choi S. M.; Shin J. H.; Kang K. K.; Ahn B. O.; Yoo M. Dig. Dis. Sci. 2007 52, 3075–3080.
• 7. Lee S. H.; Bae E. A.; Park E. K.; Shin Y. W.; Baek N. I.; Han E. J.; Chung H. G.; Kim D. H. Int. Immunopharmacol. 2007, 7 1678–1684.
• 8. Lee H. M.; Jang H. J.; Kim S. S.; Kim H. J.; Lee S. Y.; Oh M. Y.; Kwan H. C.; Jang D. S.; Eom D. W. Transplant. Proc. 2016, 48, 1226–1233.
• 9. Jeong E. K.; Jang H. J.; Kim S. S.; Oh M. Y.; Lee D. H.; Eom D. W.; Kang K. S.; Kwan H. C.; Ham J. Y.; Park C. S.; Jang D. S.; Han D. J. Transplant. Proc. 2015, 47, 757–762.
• 10. Cai M.; Phan P. T.; Hong J. G.; Kim D. H.; Kim J. M.; Park S. J.; Liu X.; Han J. E.; Park H.; ChoiJ. W.; RyuJ. H. Eur. J. Pharmacol. 2012, 689, 104–110.
• 11. U.S. Department of Health and Human Services Food and Drug Administration Draft https://www.fda.gov/downloads/Drugs/Guidances/ucm368107.pdf, September 2013.
• 12. EMEA/CHMP/EWP/192217/2009 Committee for Medicinal Products for Human Use (CHMP) http://www.ema.europa.eu/docs/en_GB/document_library/Scientific_guideline/2011/08/WC500109686.pdf, July 2011.
• 13. Wang S.; Wu H.; Geng P.; Lin Y.; Liu Z.; Zhang L.; Ma J.; Zhou Y.; Wang X.; Wen C. Biomed. Chromatogr. 2016, 30 1145–1149.
• 14. Wen C.; Wang S.; Huang X.; Liu Z.; Lin Y.; Yang S.; Ma J.; Zhou Y.; Wang X. Biomed. Chromatogr. 2015, 29 1805–1810.
• 15. Wang X.; Wang S.; Ma J.; Ye T.; Lu M.; Fan M.; Deng M.; Hu L.; Gao Z. J. Pharm. Biomed. Anal. 2015, 115, 368–374.
• 16. Ma J. S.; Wang S. H.; Huang X. L.; Geng P. W.; Wen C. C.; Zhou Y. F.; Yu L. S.; Wang X. Q. J. Pharm. Biomed. Anal. 2015, 111, 131–137.
• 17. Wen C.; Zhang Q.; He Y.; Deng M.; Wang X.; MaJ. Acta. Chromatogr. 2015, 1, 1–11.
• 18. Wen C. C.; Lin C. L.; Cai X. J.; Ma J. S.; Wang X. Q. J. Chromatogr. B. 2014, 944, 35–38.
• 19. Wang S. H.; Wu H. Y.; Huang X. L.; Geng P. W.; Wen C. C.; Ma J. S.; Zhou Y. F.; Wang X. Q. J. Chromatogr. B. 2015, 990, 118–124.
• 20. Zhang Q. W.; WenC. C.; XiangZ.; MaJ. S.; WangX. Q. J. Pharm. Biomed. Anal. 2014, 90, 134–138.
• 21. Wen C. C.; Wang S. H.; Huang X. L.; Liu Z. Z.; Lin Y. Y.; Yang S. P.; Ma J. S.; Zhou Y. F.; Wang X. Q. Biomed. Chromatogr. 2015, 29, 1805–1810.
• 22. Wang X.; Wang S.; Lin F.; Zhang Q.; Chen H.; Wang X.; Wen C.; Ma J.; Hu L. J. Chromatogr. B. 2015, 983–984, 125–131.
• 23. Ma J.; Cai J.; Lin G.; Chen H.; Wang X.; Wang X.; Hu L. J. Chromatogr. B. 2014, 959, 10–15.
• 24. Tian W.; Cai J.; Xu Y.; Luo X.; Zhang J.; Zhang Z.; Zhang Q.; Wang X.; Hu L.; Lin G. Int. J. Clin. Exp. Med. 2015, 8, 15164–15172.
• 25. Williams J. S.; Donahue S. H.; Gao H.; Brummel C. L. Bioanalysis 2012, 4, 1025–1037.
• 26. Yang Y.; Liu C.; Zhang Y.; Zhou L.; Zhong D.; Chen X. J. Pharm. Biomed. Anal. 2015, 114, 408–415.
• 27. Deng P.; Ji C.; Dai X.; Zhong D.; Ding L.; Chen X. J. Chromatogr. B. 2015, 989, 71–79.
• 28. Ji H. Y.; Lee H. W.; Shim H. J.; Kim S. H.; Kim W. B.; Lee H. S. Biomed. Chromatogr. 2004, 18, 173–177.
• 29. Kim S. H.; Kim S. H.; LeeS. D.; Kim W. B.; LeeM. G.; Kim N. D. Res. Commun. Mol. Pathol. Pharmacol. 1997, 97, 165–170.
• 30. Jang J. M.; Park K. J.; Kim D. G.; Shim H. J.; Ahn B. O.; Kim S. H.; Kim W. B. J. Appl. Pharm. 2003, 11, 163–168.

Uwagi

PL

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2018).