Bioaccumulation of Chromium, Iron, and the Expression of TNF-α and Caspase-3 in Mudskipper (Periophthalmus spp.) from Ambon Island Waters, Indonesia

• Autorzy: Liline Sintje , Rumahlatu Dominggus , Salmanu Sriyanti I. A. , Pattipeilohy Mery , Sangur Kristin

Identyfikatory

DOI

10.12911/22998993/149583

• Języki publikacji: EN

Abstrakty

EN

TNF-α protein and caspase-3 in mudskipper muscle can be used as a biomarker to evaluate the environmental conditions. Therefore, this study aimed to analyze the heavy metal contents, specifically Cr and Fe, in the sediments from the mangrove area, as well as the expression of TNF-α and caspase-3 in mudskipper muscle. The samples used were the sediments and mudskippers from the waters of Poka, Waai, and Rutong Villages. The heavy metals were analyzed using Atomic Absorption Spectrophotometry (AAS), while the TNF-α and caspase-3 expressions were assessed with immunohistochemical staining. Observations were carried out using an optical microscope with 1000x magnification. The results showed that the proportion of Fe accumulated in mudskipper at the three locations was greater than Cr, while Cr was higher in the sediment compared to Fe. Moreover, the TNF-α and caspase-3 expressions appeared in muscle organs in varying amounts. This indicates that Cr and Fe under excessive conditions can be toxic to mudskipper causing muscle inflammation and cell death, which leads to the expression of TNF-α and caspase-3.

Słowa kluczowe

EN

heavy metals; Cr; Fe; TNF-α; caspase-3; mudskipper

• Wydawca: Polskie Towarzystwo Inżynierii Ekologicznej ; Lublin University of Technology
• Czasopismo: Journal of Ecological Engineering
• Rocznik: 2022
• Tom: Vol. 23, nr 7
• Strony: 90--104
• Opis fizyczny: Bibliogr. 108 poz., rys., tab.

Twórcy

autor

Liline Sintje

• Study Program of Biology Education, Faculty of Teacher Training and Education, Pattimura University, Jl. Ir. M. Putuhena, Ambon 97233, Indonesia

• https://orcid.org/0000-0003-4973-9490

autor

Rumahlatu Dominggus

• Study Program of Biology Education, Faculty of Teacher Training and Education, Pattimura University, Jl. Ir. M. Putuhena, Ambon 97233, Indonesia

• https://orcid.org/0000-0002-4466-5528

autor

Salmanu Sriyanti I. A.

• Study Program of Biology Education, Faculty of Teacher Training and Education, Pattimura University, Jl. Ir. M. Putuhena, Ambon 97233, Indonesia

• https://orcid.org/0000-0002-2713-598X

autor

Pattipeilohy Mery

• Study Program of Biology Education, Faculty of Teacher Training and Education, Pattimura University, Jl. Ir. M. Putuhena, Ambon 97233, Indonesia

• https://orcid.org/0000-0002-1011-2503

autor

Sangur Kristin

• Study Program of Biology Education, Faculty of Teacher Training and Education, Pattimura University, Jl. Ir. M. Putuhena, Ambon 97233, Indonesia

• https://orcid.org/0000-0003-4576-6328

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