Effect of temperature, concentration of alcohols and time on baker’s yeast permeabilization process

• Autorzy: Trawczyńska I. , Miłek J. , Kwiatkowska-Marks S.
• Języki publikacji: EN

Abstrakty

EN

Baker’s yeast beyond the traditional use in the food industry may be used to carry out biotransformations. The effectiveness of yeast as biocatalysts is based on the presence of large amounts of intracellular enzymes, whose efficiency can be repeatedly increased by permeabilization. It is the process of increasing the permeability of cell walls and membranes in order to facilitate reagents access to the intracellular enzyme. Alcohols permeabilization process allows for approx. 50-fold increase in catalase activity of baker’s yeast. In this paper, the influence of physical and chemical parameters on the effectiveness of permeabilization of baker’s yeast cells using alcohols was analyzed. Research has shown that with increasing temperature of permeabilization process better results are achieved using a lower alcohol concentration. Based on presented response surface graphs, we can also indicate a negligible impact of duration time on the process efficiency.

Słowa kluczowe

EN

permeabilization; baker’s yeast; biocatalyst; response surface methodology

• Wydawca: Wydawnictwo Uniwersytetu Warmińsko-Mazurskiego w Olsztynie
• Czasopismo: Technical Sciences / University of Warmia and Mazury in Olsztyn
• Rocznik: 2018
• Tom: nr 21(3)
• Strony: 195--206
• Opis fizyczny: Bibliogr. 18 poz., tab., wykr.

Twórcy

autor

Trawczyńska I.

• Zakład Inżynierii Chemicznej i Bioprocesowej, Wydział Technologii i Inżynierii Chemicznej, Uniwersytet Techniczno-Przyrodniczy im. Jana i Jędrzeja Śniadeckich, ul. Seminaryjna 3, 85-326 Bydgoszcz

autor

Miłek J.

• Department of Chemical and Biochemical Engineering Jan and Jędrzej Śniadecki University of Technology and Life Sciences in Bydgoszcz, Poland

autor

Kwiatkowska-Marks S.

• Department of Chemical and Biochemical Engineering Jan and Jędrzej Śniadecki University of Technology and Life Sciences in Bydgoszcz, Poland

Bibliografia

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