Catechin-molecularly imprinted cryogel for determination of catechin in red wines by HPLC–DAD–fluorescence detector

• Autorzy: Büyüktuncel E. , Porgalı E. , Özkara S.

Identyfikatory

DOI

10.1556/1326.2016.00193

• Języki publikacji: EN

Abstrakty

EN

A new molecularly imprinted polymer (MIP) was prepared by using catechin (C) as the template molecule. The polymer was characterized by swelling tests, scanning electron microscopy (SEM), Brunauer–Emmett–Teller (BET), and Fourier transform infrared (FTIR) spectroscopy. The MIP with high recovery was selected as a solid-phase extraction (SPE) sorbent in this work. The standard solutions were directly applied onto the SPE cartridges following loading, washing, and elution procedures. A solution of the collected fractions was analyzed by high-performance liquid chromatography–diode-array detection (DAD) and fluorescence detector. The optimization of the method and validation was achieved on a C18 column (5 μm, 250 × 4.6 mm) with methanol–water (35:65, v/v) mixture adjusted pH 2.5 as the mobile phase at a flow rate of 1 mL min−1 at room temperature. The selectivity coefficient (k) of imprinted p(HEMA–MAH) cryogel was 5.1-fold that of non-imprinted cryogel. It showed good selectivity and affinity for C molecule. A comparison was made between the results obtained with the MIP cartridges and a traditional C18 reversed-phase cartridge. It was observed that 2.3 times higher recovery of C can be obtained on catechin-MIP cryogel. The results of the presented work showed that the prepared MIP can be used as SPE sorbent for extracting of C from red wines.

Słowa kluczowe

EN

Molecularly imprinted polymers; HPLC–DAD–fluorescence detector; catechin; red wine

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2018
• Tom: Vol. 30, no. 1
• Strony: 54--61
• Opis fizyczny: Bibliogr. 18 poz., rys., tab

Twórcy

autor

Büyüktuncel E.

• Department of Analytical Chemistry, Faculty of Pharmacy, İnönü University, 44280, Malatya, Turkey

autor

Porgalı E.

• Department of Analytical Chemistry, Faculty of Pharmacy, İnönü University, 44280, Malatya, Turkey

autor

Özkara S.

• Department of Chemistry, Biochemistry Division, Anadolu University, 26470, Eskişehir, Turkey

Bibliografia

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Uwagi

PL

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2018).