Comparative study of RP-HPLC versus TLC-spectrodensitometric methods applied for binary mixtures of fluoroquinolones and corticosteroids

• Autorzy: Elgizawy S. M. , Hassan N. Y. , Lotfy H. M. , Saleh S. S.
• Języki publikacji: EN

Abstrakty

EN

Reversed phase high-performance liquid chromatography (RP-HPLC) and thin-layer chromatography (TLC)-spectrodensitometric methods have been developed and validated for the separation and quantitation of two binary mixtures: Ofloxacin (OFX) and dexamethasone (DXM) in eye preparation; ciprofloxacin hydrochloride (CIP) and hydrocortisone (HYD) in ear preparation. The linearity ranges of RP-HPLC methods were found to be (2.5–45 μg mL^-1) for OFX, (2.5–50 μg mL^-1) for DXM and (1–8 μg mL^-1) for both CIP and HYD. The percentage recoveries/relative standard deviation (RSD) were found to be 100.36/1.38, 100.13/1.49, 99.98/0.61 and 100.28/1.27, respectively. The linearity ranges of TLC-spectrodensitometric methods were found to be (0.5–2 μg band^-1), (0.5–3.5 μg band^-1), (0.2–1.6 μg band^-1), and (0.6–2 μg band^-1 for OFX, DXM, CIP, and HYD, respectively. The percentage recoveries/RSD were found to be 99.98/1.06, 99.93/1.18, 99.74/1.27, and 99.94/1.54, respectively. A comparative study was conducted to show the advantages of the proposed methods which showed that the TLC-spectrodensitometric methods were simpler, more sensitive, and economic, while RP-HPLC methods were more precise and robust. The methods were validated in compliance with the ICH guidelines and were successfully applied for determination of the selected drugs in their laboratory-prepared mixtures and commercial dosage forms.

Słowa kluczowe

EN

ofloxacin; dexamethasone; ciprofloxacin hydrochloride; hydrocortisone; HPLC

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2014
• Tom: Vol. 26, no. 3
• Strony: 439--456
• Opis fizyczny: Bibliogr. 31 poz., rys., tab.

Twórcy

autor

Elgizawy S. M.

• Assuit University Analytical Chemistry Department, Faculty of Pharmacy 71515 Assuit Egypt

autor

Hassan N. Y.

• Cairo University Analytical Chemistry Department, Faculty of Pharmacy Kasr-El Aini Street 11562 Cairo Egypt

autor

Lotfy H. M.

• Cairo University Analytical Chemistry Department, Faculty of Pharmacy Kasr-El Aini Street 11562 Cairo Egypt

autor

Saleh S. S.

• October University for Modern Sciences and Arts (MSA) Analytical Chemistry Department, Faculty of Pharmacy 11787 6th October City Egypt

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