High performance liquid chromatography based quantification of reserpine in Rauwolfia tetraphylla L. and enhanced production through precursor feeding

• Autorzy: Rohela Gulab Khan , Bylla Prasad , Pendli Sreenu , Korra Rajender , Gandu Rajender , Reuben Christopher

Identyfikatory

DOI

10.1556/1326.2021.00888

• Języki publikacji: EN

Abstrakty

EN

Rauwolfia tetraphylla L., is an important medicinal plant in Apocynaceae family and is recognized as an alternative source to Rauwolfia serpentina L., in terms of anti-hypertensive alkaloid production i.e. reserpine. In view of this, the present study is conducted to estimate the reserpine content in different parts (leaf, stem and root) of field grown plants (2 years old), tissue cultured plantlets (R1) (two months old) and cell suspensions cultures (two months old with and without precursor feeding) of R. tetraphylla by using high performance liquid chromatography (HPLC) technique. Overall maximum content of reserpine (in %) was estimated from the root samples. Roots of field grown plants has recorded high percent of reserpine (0.39%) followed by roots of tissue cultured plantlets (0.35%) and root callus based cell suspension cultures (0.38 %) which was fed with precursor amino acid (100 mg/L of tryptophan). In control type of root callus based cell suspension cultures, reserpine content was quantified as 0.14%; by precursor feeding (100 mg/L of tryptophan) it was enhanced to 0.38%. In conclusion, the reserpine content (0.35 and 0.38%) produced by the roots of tissue cultured plantlets (R1) and 100 mg/L tryptophan fed root callus based cell suspensions was comparable to that of the reserpine content (0.39%) of root parts of field grown plants. The present study demonstrates the reserpine production by in vitro cell suspension cultures throughout the year without sacrificing the medicinal plants.

Słowa kluczowe

EN

antihypertensive; cell suspension; high performance liquid chromatography; HPLC; Rauwolfia tetraphylla; reserpine; tryptophan

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2022
• Tom: Vol. 34, no. 2
• Strony: 120--129
• Opis fizyczny: Bibliogr. 44 poz., rys., tab.

Twórcy

autor

Rohela Gulab Khan

• Department of Biotechnology, Kakatiya University, Warangal, Telangana 506 009, India
• Central Sericultural Research & Training Institute, Central Silk Board, Ministry of Textiles, Govt. of India, Pampore 192 121, Jammu & Kashmir, India

• https://orcid.org/0000-0002-3882-1547

autor

Bylla Prasad

• Department of Biotechnology, Kakatiya University, Warangal, Telangana 506 009, India

autor

Pendli Sreenu

• Department of Botany, Kakatiya University, Warangal, Telangana 506 009, India

autor

Korra Rajender

• Department of Botany, Kakatiya University, Warangal, Telangana 506 009, India

autor

Gandu Rajender

• Department of Botany, Kakatiya University, Warangal, Telangana 506 009, India

autor

Reuben Christopher

• Department of Botany, Kakatiya University, Warangal, Telangana 506 009, India

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Uwagi

Opracowanie rekordu ze środków MEiN, umowa nr SONP/SP/546092/2022 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2022-2023).