Isolation and Purification of Isochlorogenic Acid A from Lonicera japonica Thunb Using High-Speed Counter-Current Chromatography

• Autorzy: Yu X. X. , Wu J.-W. , Liang Z. K. , Zhao M. , Xu X. J.
• Języki publikacji: EN

Abstrakty

EN

A high-speed counter-current chromatography (HSCCC) method was established for the isolation and purification of isochlorogenic acid A from Lonicera japonica Thunb. The two-phase solvent system was composed of n-hexane:ethyl acetate: isopropanol:water (2:3:2:5, v/v/v/v). From 150 mg of the ethyl acetate fraction of L. japonica Thunb, 19.65 mg of isochlorogenic acid A was obtained in a one-step HSCCC separation, with a purity of 99.1%, as determined by high-performance liquid chromatography (HPLC). The structure was further identified by ultraviolet (UV), mass spectrometry (MS) and nuclear magnetic resonance (NMR).

Słowa kluczowe

EN

Lonicera japonica Thunb; isochlorogenic acid A; HSCCC

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2015
• Tom: Vol. 27, no. 3
• Strony: 541--549
• Opis fizyczny: Bibliogr. 10 poz., rys., tab.

Twórcy

autor

Yu X. X.

• School of Pharmaceutical Sciences, Sun Yat-sen University, No. 132 East Waihuan Rd., Guangzhou Higher Education Mega Center, Guangzhou 510006, China
• Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, China

autor

Wu J.-W.

• School of Pharmaceutical Sciences, Sun Yat-sen University, No. 132 East Waihuan Rd., Guangzhou Higher Education Mega Center, Guangzhou 510006, China
• Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, China

autor

Liang Z. K.

• School of Pharmaceutical Sciences, Sun Yat-sen University, No. 132 East Waihuan Rd., Guangzhou Higher Education Mega Center, Guangzhou 510006, China
• Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, China

autor

Zhao M.

• School of Pharmaceutical Sciences, Sun Yat-sen University, No. 132 East Waihuan Rd., Guangzhou Higher Education Mega Center, Guangzhou 510006, China
• Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, China

autor

Xu X. J.

• School of Pharmaceutical Sciences, Sun Yat-sen University, No. 132 East Waihuan Rd., Guangzhou Higher Education Mega Center, Guangzhou 510006, China
• Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, China

Bibliografia

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