Effectiveness of Intrinsic Biodegradation Enhancement in Oil Hydrocarbons Contaminated Soil

• Autorzy: Zawierucha I. , Malina G. , Ciesielski W. , Rychter P.

Identyfikatory

DOI

10.2478/aep-2014-0010

Warianty tytułu

Efektywność wspomagania biodegradacji w gruncie zanieczyszczonym węglowodorami ropopochodnymi

• Języki publikacji: EN

Abstrakty

EN

Studies were conducted using a 10-chamber Micro-Oxymax (Columbus, OH, USA) respirometer to determine the effect of bioaugmentation, biostimulation and combination of them on enhancing intrinsic biodegradation of oil hydrocarbons in soil. Contaminated soil was collected from a former military airport in Kluczewo, Poland. Bioaugmentation was realized by addition of indigenous or exogenous bacteria to soil. Biostimulation was done by aerated water supply and surfactant addition. Bioaugmentation + addition of a surfactant was applied as the combined treatment. The intrinsic and enhanced hydrocarbons biodegradation rates were estimated from the slopes of linear regressions of cumulative curves of O₂ uptake. Pertinent biodegradation rates were recalculated on the basis of the stoichiometric reaction (mass balance equation) and conversion equation. The results showed that combined treatment (indigenous bacteria bioaugmentation + addition of a surfactant) was the most effective method of biodegradation enhancement as the 20-fold increase of biodegradation rate was observed.

PL

Przeprowadzono badania mające na celu określenie efektywności wspomagania biodegradacji węglowodorów ropopochodnych w gruncie w wyniku zastosowania bioaugmentacji, biostymulacji lub metody kombinowanej. Próbki gruntu użyte do badań zostały pobrane z terenu Centralnej Stacji Tankowania (CST) lotniska Kluczewo niedaleko Stargardu Szczecińskiego. Bioaugmentację przeprowadzono z użyciem autochtonicznych i allochtonicznych mikroorganizmów zdolnych do rozkładu węglowodorów ropopochodnych. Z kolei biostymulacja obejmowała wprowadzenie napowietrzonej wody lub substancji powierzchniowo czynnej (SPC) do zanieczyszczonego gruntu. Biodegradację węglowodorów ropopochodnych szacowano na podstawie konsumpcji O₂ przy użyciu respirometru Micro-Oxymax V6.0 COLUMBUS INSTRUMENTS. Średnie szybkości konsumpcji O₂ podczas biodegradacji węglowodorów wyznaczono z równań aproksymacji liniowej krzywych kumulacyjnych. Na podstawie równania bilansu masy i wyznaczonych szybkości konsumpcji O₂ obliczono szybkość biodegradacji węglowodorów, tj. szybkość ubytku substratu w czasie. Z przeprowadzonych badań wynika, że metoda kombinowana (kombinacja bioaugmentacji z dodatkiem SPC) była najbardziej efektywną metodą wspomagania biodegradacji węglowodorów ropopochodnych w gruncie - odnotowano wtedy 20-krotny wzrost szybkości biodegradacji.

Słowa kluczowe

EN

intrinsic biodegradation; enhanced biodegradation; biostimulation; bioaugmentation; combined enhancement

PL

biodegradacja węglowodorów; bioaugmentacja; biostymulacja; metoda kombinowana

• Wydawca: Institute of Environmental Engineering, Polish Academy of Sciences
• Czasopismo: Archives of Environmental Protection
• Rocznik: 2014
• Tom: Vol. 40, no. 1
• Strony: 101--113
• Opis fizyczny: Bibliogr. 36 poz., rys., tab., wykr.

Twórcy

autor

Zawierucha I.

• Institute of Chemistry, Environmental Protection and Biotechnology, Jan Dlugosz University of Czestochowa,Armii Krajowej 13/15, 42-200 Czestochowa, Poland

autor

Malina G.

• Department of Hydrogeology and Engineering Geology, AGH University of Science and Technology,Mickiewicza 30, 30-059 Cracow

autor

Ciesielski W.

• Institute of Chemistry, Environmental Protection and Biotechnology, Jan Dlugosz University of Czestochowa,Armii Krajowej 13/15, 42-200 Czestochowa, Poland

autor

Rychter P.

• Institute of Chemistry, Environmental Protection and Biotechnology, Jan Dlugosz University of Czestochowa,Armii Krajowej 13/15, 42-200 Czestochowa, Poland

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