Enzymatic bioconversion of feather waste with keratinases of Bacillus cereus PCM 2849

• Autorzy: Ciurko Dominika P. , Łaba Wojciech , Piegza Michał , Juszczyk Piotr , Choińska-Pulit Anna , Sobolczyk-Bednarek Justyna

Identyfikatory

DOI

10.2478/pjct-2019-0030

• Języki publikacji: EN

Abstrakty

EN

Enzymatic preparation from culture of keratinolytic Bacillus cereus PCM 2849 was applied for hydrolysis of whole chicken feathers, after sulphitolytic pretreatment. This process was optimized using a three-factor Box-Behnken design, where the effect of substrate concentration, sulphite concentration during pretreatment and reaction temperature was evaluated on the release of amino acids. Obtained results revealed the highest impact of reaction temperature, followed by substrate content and sulphite during pretreatment. Optimal process conditions were established, i.e. temperature 44.4°C, feathers 4.7% and treatment with 25.3 mM sulphite. Amino acid composition of the obtained hydrolysate was analyzed. Glutamic acid (9.21 g·kg⁻¹) and proline were dominant, however significant amount of branched-chain amino acids was also observed. The FTIR analysis of residual substrate revealed the cleavage of disulphide bonds in keratin through the presence of thioester residues. The absence of reduced cysteine residues was confirmed, along with minor changes in proportions of keratin substructures.

Słowa kluczowe

EN

feather waste; Bacillus cereus; Box-Behnken design; hydrolysis; thermo-chemical pretreatment

• Wydawca: West Pomeranian University of Technology. Publishing House
• Czasopismo: Polish Journal of Chemical Technology
• Rocznik: 2019
• Tom: Vol. 21, nr 3
• Strony: 53--59
• Opis fizyczny: Bibliogr. 26 poz., rys., tab.

Twórcy

autor

Ciurko Dominika P.

• Wrocław University of Environmental and Life Sciences, Department of Biotechnology and Food Microbiology, Faculty of Biotechnology and Food Science, J. Chełmońskiego 37, 51-630 Wrocław; Poland

autor

Łaba Wojciech

• Wrocław University of Environmental and Life Sciences, Department of Biotechnology and Food Microbiology, Faculty of Biotechnology and Food Science, J. Chełmońskiego 37, 51-630 Wrocław; Poland

autor

Piegza Michał

• Wrocław University of Environmental and Life Sciences, Department of Biotechnology and Food Microbiology, Faculty of Biotechnology and Food Science, J. Chełmońskiego 37, 51-630 Wrocław; Poland

autor

Juszczyk Piotr

• Wrocław University of Environmental and Life Sciences, Department of Biotechnology and Food Microbiology, Faculty of Biotechnology and Food Science, J. Chełmońskiego 37, 51-630 Wrocław; Poland

autor

Choińska-Pulit Anna

• “Poltegor-Institute”, Opencast Mining Institute, Parkowa 25, 51-616 Wrocław, Poland

autor

Sobolczyk-Bednarek Justyna

• “Poltegor-Institute”, Opencast Mining Institute, Parkowa 25, 51-616 Wrocław, Poland

Bibliografia

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Uwagi

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2019).