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Języki publikacji
Abstrakty
The aim of this study was to develop and validate a HPLC-MS/MS assay to determine the lutein concentration in plasma samples of human and SD rats. Organic solvent was used for lutein extraction. The extract was injected into a HPLC-MS/MS system. Reversed phase chromatography was performed on a C18 column in gradient mode. Lutein and internal standard (phenytoin sodium) were identified in atmospheric pressure chemical ionization mode using ion transitions of m/z 567.5>549.4 and 205.2>110.8, respectively. The lutein quantification assay was linear over concentrations ranging from 4 to 500 ng/mL. The lower limit of quantification was 4 ng/mL with satisfactory precision and accuracy. The assay presented acceptable intra and inter-batch precision (RSD%) and accuracy (RE%) <8.16% in SD rat plasma and <12.80% in human plasma. The extraction recovery ranged from 50.94 to 60.90% in SD rat plasma and 68.73% in human plasma. The matrix effect for lutein was acceptable and had minimal influence on the results. The method was then applied to determine the lutein concentrations in human plasma after a single oral dose of 20mg lutein. The method described is rapid, selective, sensitive and reproducible. This method can be used for both pharmacokinetic studies and therapeutic drug monitoring purposes.
Słowa kluczowe
Czasopismo
Rocznik
Tom
Strony
19--22
Opis fizyczny
Bibliogr. 15 poz., rys., tab.
Twórcy
autor bibliografii
- National Institute for Drug Clinical Trial, Beijing Tongren Hospital, Capital Medical University, Beijing-100730, China
autor
- National Institute for Drug Clinical Trial, Beijing Tongren Hospital, Capital Medical University, Beijing-100730, China
autor
- National Institute for Drug Clinical Trial, Beijing Tongren Hospital, Capital Medical University, Beijing-100730, China
autor
- National Institute for Drug Clinical Trial, Beijing Tongren Hospital, Capital Medical University, Beijing-100730, China
autor
- National Institute for Drug Clinical Trial, Beijing Tongren Hospital, Capital Medical University, Beijing-100730, China
autor
- National Institute for Drug Clinical Trial, Beijing Tongren Hospital, Capital Medical University, Beijing-100730, China
autor
- National Institute for Drug Clinical Trial, Beijing Tongren Hospital, Capital Medical University, Beijing-100730, China
autor
- Zhejiang Medicine Co. Ltd, Xinchang Pharma. Factory, Zhejiang Xinchang-312500, China
autor
- Zhejiang Medicine Co. Ltd, Xinchang Pharma. Factory, Zhejiang Xinchang-312500, China
Bibliografia
- [1] Johnson, E. J.; Maras, J. E.; Rasmussen, H. M.; Tucker, K. L. J. Am. Diet. Assoc. 2010, 110, 1357–1362.
- [2] Landrum, J. T.; Bone, R. A.; Moore, L. L.; Gomez, C. M. Methods Enzymol. 1999, 299, 457–467.
- [3] Chiu, C. J.; Taylor, A. Exp. Eye Res. 2007, 84, 229–245.
- [4] García-Layana, A.; Recalde, S.; Alamán, A. S.; Robredo, P. F. Nutrients 2013, 5, 543–551.
- [5] Ma, L.; Yan, S. F.; Huang, Y. M.; Lu, X. R.; Qian, F.; Pang, H. L.; Xu, X. R.; Zou, Z. Y.; Dong, P. C.; Xiao, X.; Wang, X.; Sun, T. T.; Dou, H. L.; Lin, X. M. Ophthalmology 2012 , 119, 2290–2297.
- [6] Murray, I. J.; Makridaki, M.; van der Veen, R. L.; Carden, D.; Parry, N. R.; Berendschot, T. T. Invest. Ophthalmol. Vis. Sci. 2013, 54, 1781–1788.
- [7] Huang, Y. M.; Dou, H. L.; Huang, F. F.; Xu, X. R.; Zou, Z. Y.; Lu, X. R.; Lin, X. M. Br. J. Ophthalmol. 2015, 99, 371–375.
- [8] Thürmann, P. A.; Schalch, W.; Aebischer, J. C.; Tenter, U.; Cohn, W. Am. J. Clin. Nutr. 2005, 82, 88–97.
- [9] Schalch, W.; Cohn, W.; Barker, F. M.; Kõpcke, W.; Mellerio, J.; Bird, A. C.; Robson, A. G.; Fitzke, F. F.; van Kuijk, Arch. Biochem. Biophys. 2007, 458, 128–135.
- [10] Obana, A.; Tanito, M.; Gohto, Y.; Okazaki, S.; Gellermann, W.; Bernstein, P. S. PLoS One 2015, 10, e0139257.
- [11] Evans, M.; Beck, M.; Elliott, J.; Etheve, S.; Roberts, R.; Schalch, W. Eur. J. Nutr. 2013, 52,1381–1391.
- [12] Hartmann, D.; Thürmann, P. A.; Spitzer, V.; Schalch, W.; Manner, B.; Cohn, W. Am. J. Clin. Nutr. 2004, 79, 410–417.
- [13] Sato, Y.; Kobayashi, M.; Itagaki, S.; Hirano, T.; Noda, T.; Mizuno, S.; Sugawara, M.; Iseki, K. Biopharm. Drug Dispos. 2011, 32, 151–158.
- [14] Sowmya, P. R.; Arathi, B. P.; Vijay, K.; Baskaran, V.; Lakshminarayana, R. Chromatographia 2014, 77, 1633–1642.
- [15] Guidance for Industry: Bioanalytical Method Validation, U.S. Department of Health and Human Service, Food and Drug Administration, Center for Drug Evaluation and Research (CDER), Centre for Veterinary Medicine (CVM), May 2001.
Uwagi
PL
Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2019).
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-7989afa7-ed85-48c0-9565-f654d066f548