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Filamentous bacteria in activated sludge - review of morphotypes, the morphological and molecular criteria
Języki publikacji
Abstrakty
Bakterie nitkowate w osadzie czynnym tworzą szkielet kłaczków i rozkładają polimery zawarte w ściekach. Przy ich nadmiernym wzroście zmienia się struktura kłaczków i pojawiają się problemy eksploatacyjne - pienienie i pęcznienie osadu. Skuteczne zwalczanie tych zjawisk jest łatwiejsze, gdy uda się zidentyfikować organizmy za nie odpowiedzialne. Klasyczna analiza mikroskopowa pozwala określić jedynie morfotyp bakterii nitkowatych. Metoda ta jest niedoskonała, gdyż dany morfotyp może obejmować kilka typów filogenetycznych o różnych właściwościach ekofizjologicznych, co utrudnia zwalczanie bakterii odpowiedzialnych za pęcznienie osadu. W celu właściwej identyfikacji należy wykorzystać techniki biologii molekularnej, jak np. metodę fluorescencyjnej hybrydyzacji in situ (FISH).
Filamentous bacteria form the backbone of flocs in activated sludge and decompose polymers in wastewater. Their excessive growth results in changes in the structure of the flocs and operational problems - bulking and foaming. The effective control of these phenomena requires a reliable identification of microorganisms causing these problems. Conventional microscopic analysis of activated sludge leads only to a morphotype classification. This method is not perfect because a morphotype may include several phylogenetic ecotypes with different ecophysiological properties. Therefore, the control of bacteria growth responsible for sludge bulking is difficult. Molecular biology techniques, e.g. the method of fluorescence in-situ hybridisation (FISH), should be used for reliable identification.
Słowa kluczowe
Rocznik
Tom
Strony
73--92
Opis fizyczny
Bibliogr. 33 poz., tab.
Twórcy
autor
- Zakład Biologii, Wydział Inżynierii Środowiska PW
autor
- Zakład Biologii, Wydział Inżynierii Środowiska PW
Bibliografia
- [1] Beer M., Seviour E.M., Kong Y., Cunningham M., Blackall L.L., Seviour R.J. (2002): Phylogeny of the filamentous bacterium Eikelboom Type 1851, and design and application of 16S rRNA targeted oligonucleotide probe for its fluorescence in situ identification in activated sludge. FEMS Microbiol. Left. 207: 179-183.
- [2] Bjornsson L., Hugenholtz P., Tyson G.W., Blackall L.L. (2002): Filamenotus Chloroflexi (green non-sulfur bacteria) are abundant in wastewater treatment process with biological nutrient removal. Microbiology 148: 2309-2318.
- [3] Blackall L.L., Seviour E.M., Bradform M., Rossetti S., Tandoi V., Seviour R.J. (2000): 'Candidatus Nostocoida limicola ', a filamentous bacterium from activated sludge. International Journal of Systematic and Evolutionary Microbiology 50: 703-709.
- [4] Davenport R.J., Curtis T.P., Goodfellow M., Stainsby F.M., Bingley M. (2000): Quantitive use of fluorescent in situ hybridization to examine relationship between mycolic acid-containing actinomycetes and foaming in activated sludge plants. Appl. Environ. Microbial. 66: 1158-1166.
- [5] Eikelboom D.H. (1975): Filamentous organisms observed in activated sludge. Water Res. 9: 365-388.
- [6] Eikelboom D.H. (2000): Process control of activated sludge plants by microscopic investigation. IWA Publishing, London, England.
- [7] Erhart R., Bradford D., Seviour R.J., Amann R., Blackall L.L. (1997): Development and use of fluorescent in situ hybridization probes for the detection and identification of "Microthrix parvicella" in activated sludge. Syst. Appl. Microbiol. 20: 310-318.
- [8] Fialkowska E., Fyda J., Pajdak-Stoś A., Wiąckowski K. (2010): Osad czynny: biologia i analiza mikroskopowa, Wydawnictwo Seidel i Przywecki Sp. z o.o., Piaseczno.
- [9] Howarth R., Head I.M., Unz R.F. (1998): Phylogenetic assessment of five filamentous bacteria isolated from bulking activated sludge. Water Sci. Technol. 37: 303-306.
- [10] Hugenholtz P., Tyson G.W., Webb R.I., Wagner A.M., Blackall L.L.(2001): Investigation of candidate division TM7, a recently recognized major linage of the domain Bacteria with no known pure-culture representatives. Applied and Environmental Microbiology 67: 411-419.
- [11] Jenkins D., Richard M., Daigger G. (1993): Manual on Causes and Control of Activated Sludge Bulking and Foaming, Chelsea, Michigan: Lewis Publisher
- [12] Jenkins D., Richard M.G., Daigger G.T. (2004). Manual on Causes and Control of Activated Sludge Bulking, Foaming, and other solids separation problems. IWA Publishing, CRC Press, London UK.
- [13] Kanagawa T., Kamagata Y., Aruga S., Kohno T., Horn M., Wagner M. (2000): Phylogenetic analysis of and oligonucleotide probe development for eikelboom type 021N filamentous bacteria isolated from bulking activated sludge. Appl. Eniviron. Microbiol. 66: 5043-5052.
- [14] Kohno T., Sei K., Mori K. (2002): Characterization of Type 1851 organism isolated from activated sludge samples. Water Sci. Technol. 46: 111-114.
- [15] Kragelund C., Levantesi C., Borger A., Thelen K., Eikelboom D., Thandoi V., Kong Y., van der Waarde Y., Krooneman J, Rossetti S., Roughed T., Thornsen T.R., Nielsen P.H. (2007): Identity, abundance and ecophysiology of filamentous Chloroflexi species present in activated sludge treatment plants. FEMS Microbiology Ecology 59: 671-682.
- [16] Kragelund C., Remesova Z., Nielsen J.L., Thomsen T.R., Eales K.I., Seviour R.J., Wanner J., Nielsen P.H. (2007): Ecophysiology of mycolic acid containing Actinobacteria (Mycolata) in activated sludge foams. FEMS Microbiol. Ecol. 61: 174-184.
- [17] Kragelund C., Levantesi C., Borger A., Thelen K., Eikelboom D., Thandoi V., Kong Y., KroonemanJ., Larsen P., Thomsen T.R., Nielsen P.R. (2008): Identity, abundance and ecophysiology of filamentous bacteria belonging to Bacteroidetes present in activated sludge plants. Microbiology 154: 886-894.
- [18] Lemmer H. (2000): Przyczyny powstawania i zwalczanie osadu spęczniałego, Wydawnictwo Seidel-Przywecki Sp. z o.o., Szczecin.
- [19] Levantesi C., Rossetti S., Thelen K., Kragelund C., Krooneman J., Eikelboom D., Nielsen PH, Tandoi V. (2006): Phylogeny, physiology and distribution of 'Candidatus Microthrix calida', a new Microthrix species isolated from industrial activated sludge wastewater treatment plants. Environ. Microbiol. 8: 1552-1563.
- [20] Liu J.R., McKenzie C.A., Seviour E.M., Webb R.I., Blackall L.L., Saint C.P., Seviour R.J. (2001): Phylogeny of the filamentous bacterium 'Nostocoida limicola ' III from activated sludge. International Journal of Systematic and Evolutionary Microbiology 51: 195-202.
- [21] Macadaly J.L., Lyou E.H., Koffman B., Albertson L.K., Meyer K., Galdenzi S., Mariani F. (2006): Dominant microbial populations in limestone-corroding stream biofilms, Frasassi cave system, Italy, Appl. Environ. Microbiol. 72: 5569-5609.
- [22] Mussmann M., Schulz H.N, Strotmann B., Kjaer T., Nielsen L.P., Rosselló-Mora R.A., Amann R.I., Jorgensen B.B. (2003): Phylogeny and distribution of nitrate-storing Beggiatoa spp. in coastal marine sediments. Environ. Microbiol. 5: 523-533.
- [23] Müller E., Schade M., Lemmer H. (2007): Filamentous scum bacteria in activated sludge microscopy versus fluorescence in situ hybridization. Water Environ. Res. 79: 2274-2286.
- [24] Nielsen R.H., Daims H., Lemmer H. (2009): FISH handbook for biological wastewater treatment, IWA Publishing, London.
- [25] Schade M., Beimfohr C., Lemmer H. (2002): Phylogenetic and physiological characterization of a "Nostocoida limicola" - like organism isolated from activated sludge. Water Science and Technology 46(1-2): 91-97.
- [26] Schuppler M., Wagner M., Schön G., Goebel U.B. (1998): In situ identification of nocar-dioform actinomycetes in activated sludge using fluorescent rRNA-targeted oligonucleotide probes. Microbiology 144: 249-259.
- [27] Seviour E.M., Blackall L.L., Christensson C., Hugenholtz R, Cunnigham M.A., Bradford D, Stratton H.M., Seviour R.J. (1997): The filamentous morphotype Eikelboom Type 1863 is not a single genetic entity. J. Appl. Microbiol. 82: 411-421.
- [28] Speirs L., Nittani T., Mcllroy S., Schroeder S., Seviour R. J. (2009): Filamentous bacterium Eikelboom type 0092 in activated sludge plants in Australia is a member of the phylum Chloroflexi, Applied and Environmental Microbiology 75: 2446-2542.
- [29] Thomsen T.R., Nielsen J.L., Ramsing N.B., Nielsen P.H. (2004): Micromanipulation and further identification of FISH-labelled microcolonies of dominant denitryfing bacterium in activated sludge. Environ. Microbiol. 6: 470-479.
- [30] Thomsen T.R., Kragelund C., Nielsen P.H. (2006): Identity, abundance and physiology of Aquaspirillum-related filamentous bacteria in activated sludge. Water Sci. Technol. 54: 237-245.
- [31] van der Waarde J., Krooneman J., Geurkink B., van der Werf A., Eikelboom D., Beimfohr C., Snaidr J., Levantesi C., Tandoi V. (2002): Molecular monitoring of bulking sludge in industrial wastewater treatment plants. Water Sci. Technol. 46: 551-558.
- [32] Wagner M., Amann R., Kampfer P., Assmus B., Hartmann A., Hutzler P., Springer N., Schleifer K.-H. (1994a): Identification and in situ detection of Gram-negative filamentous bacteria in activated sludge. Syst. Appl. Microbiol 17: 405-417.
- [33] Wagner M., Erhart R., Manz W., Amann R., Lemmer H., Wedi D., Schleifer K.H. (1994b): Development of an rRNA-targeted oligonucleotide probe specific for the genus Actinobacter and its application for in situ monitoring in activated sludge. Syst. Appl. Microbiol 60: 792-800.
Typ dokumentu
Bibliografia
Identyfikator YADDA
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