High-performance thin-layer chromatographic analysis of psoralen in marketed formulations and manufactured solid lipid nanoparticles (SLNs): Validation of the method

• Autorzy: Akhtar N. , Faiyazuddin Md. , Mustafa G. , Sultana Y. , Baboota S. , Ali J.
• Języki publikacji: EN

Abstrakty

EN

A quantitative method using precoated silica gel-60 Lichrosphere high-performance thin-layer chromatography (HPTLC) plates, automated band wise sample application, and n-hexane:acetone:formic acid (2:1:0.025 υ/υ/υ) as mobile phase, has been developed and validated for the analysis of psoralen in marketed formulations and novel solid lipid nanoparticles (SLNs). Densitometric analysis was performed at 250 nm in absorbance mode. Compact bands of psoralen were obtained at Rf 0.32 ± 0.02. The method was validated for linearity, precision, robustness, sensitivity, specificity, and recovery. Linearity (r2 = 0.995), limit of detection (8.0 ng band^-1), limit of quantification (18.1 ng band) ^-1, recovery (98.06–99.64%), and precision (≤0.74) were satisfactory. Statistical analysis established that the developed method for quantification of psoralen in marketed formulation and from solid lipid nanoparticles is reproducible and selective.

Słowa kluczowe

EN

psoralen; Babchi oil; high-performance thin-layer chromatography; validation; solid lipid nanoparticles

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2012
• Tom: Vol. 24, no. 4
• Strony: 603--613
• Opis fizyczny: Bibliogr. 14 poz., rys., tab.

Twórcy

autor

Akhtar N.

• Hamdard University Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India

autor

Faiyazuddin Md.

• Hamdard University Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India
• Integral University Faculty of Pharmacy Lucknow 226026 Uttar Pradesh India

autor

Mustafa G.

• Hamdard University Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India

autor

Sultana Y.

• Hamdard University Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India

autor

Baboota S.

• Hamdard University Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India

autor

Ali J.

• Hamdard University Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India

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