Characterization of amino-, epoxy- and carbonyl-functionalized halloysite and its application in the immobilization of aminoacylase from Aspergillus melleus

• Autorzy: Kołodziejczak-Radzimska Agnieszka , Jesionowski Teofil

Identyfikatory

DOI

10.5277/ppmp18180

• Języki publikacji: EN

Abstrakty

EN

Functionalized halloysite was used as a support for the immobilization of an enzyme. The surface of halloysite was modified with amino (–NH), epoxy (–C(O)C) and carbonyl (–C=O) groups. Both unmodified and modified forms of the support underwent a comprehensive physicochemical and structural evaluation, including morphological, structural, thermogravimetric and spectroscopic analysis. Aminoacylase from Aspergillus melleus was used as the enzyme in the immobilization process. The process of immobilization by adsorption was performed for 1, 6 and 24 h using different concentrations of enzyme solution (0.5, 1 and 3 mg/cm3). The quantity of aminoacylase loaded onto the support was calculated by the Bradford method. Free and immobilized aminoacylase were used to catalyze the deacetylation of N-acetyl-L-methionine. Additionally, the thermal and chemical stability of the obtained biocatalytic systems were evaluated, as well as the reusability of the immobilized systems. The biocatalytic system with amino groups demonstrated activity above 70% in the pH range 4–9 and 60% in the temperature range 30–70 °C. Aminoacylase immobilized on amino-functionalized halloysite also retains around 50% of its initial activity after five reaction cycles.

Słowa kluczowe

EN

functionalized halloysite; physicochemical and structural evaluation; immobilization; thermal and chemical stability; reusability

• Wydawca: Oficyna Wydawnicza Politechniki Wrocławskiej
• Czasopismo: Physicochemical Problems of Mineral Processing
• Rocznik: 2019
• Tom: Vol. 55, iss. 1
• Strony: 128--139
• Opis fizyczny: Bibliogr. 31 poz., rys., tab.

Twórcy

autor

Kołodziejczak-Radzimska Agnieszka

• Poznan University of Technology, Faculty of Chemical Technology, Institute of Chemical Technology and Engineering, Berdychowo 4, PL-60965, Poznan, Poland

autor

Jesionowski Teofil

• Poznan University of Technology, Faculty of Chemical Technology, Institute of Chemical Technology and Engineering, Berdychowo 4, PL-60965, Poznan, Poland

Bibliografia

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Uwagi

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2019).