Isolation and Identification of Native Bacteria from Total Petroleum Hydrocarbon Polluted Soil in Wonocolo Public Oilfields, Indonesia

• Autorzy: Sari Gina Lova , Trihadiningrum Yulinah , Ni’matuzahroh

Identyfikatory

DOI

10.12911/22998993/110816

• Języki publikacji: EN

Abstrakty

EN

The presented study concerns on isolation and identification of indigenous bacteria in total petroleum hydrocarbon (TPH) polluted soil. The composite TPH polluted soil was collected from Wonocolo public oilfields, Indonesia. Pour plate and plate count techniques were used to bacterial population analysis and enumeration, respectively. Two dominant bacterial colonies were isolated from 4.06x10⁷ CFU/g population in polluted soil, then morphologically and biochemically were characterized using Microbact Identification Kits (Microbact^TM GNB12A and 12B). The identification of isolated bacteria was performed using Bergey’s Manual of Determinative Bacteriology. The results showed that the strains of bacteria are Bacillus sp. and B. cereus with probability of 72.00 and 77.00%, respectively. These strains potentially acted as biosurfactant producers and hydrocarbon degraders. Thus, biostimulation could be implemented to reduce the TPH levels in polluted soil at Wonocolo public oilfields.

Słowa kluczowe

EN

Bacillus sp.; Bacillus cereus; TPH; polluted soil; Wonocolo public oilfields

• Wydawca: Polskie Towarzystwo Inżynierii Ekologicznej ; Lublin University of Technology
• Czasopismo: Journal of Ecological Engineering
• Rocznik: 2019
• Tom: Vol. 20, nr 8
• Strony: 60--64
• Opis fizyczny: Bibliogr. 22 poz., rys., tab.

Twórcy

autor

Sari Gina Lova

• Faculty of Engineering, Universitas Singaperbangsa Karawang, Teluk Jambe Timur, Karawang 41361, Indonesia

autor

Trihadiningrum Yulinah

• Department of Environmental Engineering, Faculty of Civil Engineering, Environment, and Geoscience, Institut Teknologi Sepuluh Nopember, Surabaya 60111, Indonesia

autor

Ni’matuzahroh

• Department of Biology, Faculty of Science and Technology, Airlangga University, Surabaya 60115, Indonesia

Bibliografia

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