Analysis of Saussurea species from tibet using HPLC-DAD-ESI-MS n

• Autorzy: Dawa Z , Zhou Y. , Bai Y. , Gesang S , Liang J. , Ding L.
• Języki publikacji: EN

Abstrakty

EN

An HPLC-DAD-ESI-MS n method has been developed for simultaneous quantification of eight major compounds in eight Saussurea species which have long been used as the traditional Tibetan medicines. The method was validated for sensitivity, precision, and accuracy. LODs were from 0.11 to 5.01 μg mL ^-1, overall intra-day and inter-day variation was less than 2.70%, and overall recovery was over 98.0%. The correlation coefficients ( r ²) of the calibration plots were >0.991. This newly established method was successfully used to reveal difference among the chemical profiles and analytes contents of eight Saussurea species collected in Tibet. In addition, by comparison of UV and mass spectra with those of authentic compounds, a total of fifteen peaks were identified. It can be concluded that this is an effective method for quantification and evaluation of the flavonoids and coumarins in the eight species of the genus Saussurea . It can be used as an efficient reference method for development and use of the eight traditional Tibetan medicines by comparing their different characteristics.

Słowa kluczowe

EN

HPLC-DAD-ESI-MS n; analysis; flavonoid; coumarin; Saussurea species; Tibet

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2010
• Tom: Vol. 22, no. 1
• Strony: 141--152
• Opis fizyczny: Bibliogr. 33 poz., rys., tab.

Twórcy

autor

Dawa Z

• Tibet Autonomous Region Institute for Food and Drug Control Lhasa 850000 China
• Chinese Academy of Sciences Chengdu Institute of Biology Chengdu 610041 China

autor

Zhou Y.

• Chinese Academy of Sciences Chengdu Institute of Biology Chengdu 610041 China

autor

Bai Y.

• Tibet Autonomous Region Institute for Food and Drug Control Lhasa 850000 China

autor

Gesang S

• Tibet Autonomous Region Institute for Food and Drug Control Lhasa 850000 China

autor

Liang J.

• Chinese Academy of Sciences Chengdu Institute of Biology Chengdu 610041 China

autor

Ding L.

• Chinese Academy of Sciences Chengdu Institute of Biology Chengdu 610041 China

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