Growth of animal cells on flexible interface of liquid fluorocarbon

• Autorzy: Pilarek M. , Grabowska I.
• Języki publikacji: EN

Abstrakty

EN

The aim of this work was to study BHK-21 fibroblasts cultured on the interfacial area of two immiscible liquids: perfluorodecalin (hydrophobic; PFD) and cell culture medium (aqueous; DMEM) what allows creating 3-D multicellular structures. We showed that the robust growth of 3-D aggregated animal cells could be achieved on flexible PFD/DMEM interfacial area. We also indicated that 3-D aggregates of BHK-21 cells could be successfully subcultured on solid hydrophobic surface and cells could migrate from those multicellular structures, spread on solid surface and further grow in typical monolayered form. Results of our experiments showed that the PFD/medium system is simple and ready-to-use method without need of any inserts traditionally used for 3-D cultures of animal cells. Formed multicellular 3-D aggregates could be directly used in the same culture system for inoculation of biomaterial elements or scaffolds.

Słowa kluczowe

EN

perfluorochemical (perfluorocarbon); liquid/liquid culture system; flexible interfacial area; animal cell aggregates

• Wydawca: Polish Society for Biomaterials in Cracow
• Czasopismo: Engineering of Biomaterials
• Rocznik: 2012
• Tom: Vol. 15, no. 114
• Strony: 12--14
• Opis fizyczny: Bibliogr. 14 poz., rys.

Twórcy

autor

Pilarek M.

• Warsaw University of Technology, Faculty of Chemical and Process Engineering, Department of Biotechnology and Bioprocess Engineering, ul. Waryńskiego 1, 00-645 Warsaw, Poland

autor

Grabowska I.

• University of Warsaw, Faculty of Biology, Institute of Zoology, Department of Cytology, ul. Miecznikowa 1, 02-096 Warsaw, Poland

Bibliografia

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• [3] Piersma A.H.: Validation of alternative methods for developmental toxicity testing. Toxicology Letters 149 (2004) 147-153.
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• [7] Pilarek M., Glazyrina J., Neubauer P.: Enhanced growth and recombinant protein production of Escherichia coli by a perfluorinated oxygen carrier in miniaturized fed-batch cultures. Microbial Cell Factories 10 (2011) 50.
• [8] Pilarek M., Szewczyk K.W.: Effects of perfluorinated oxygen carrier application in yeast, fungi and plant cell suspension cultures. Biochemical Engineering Journal 41 (2008) 38-42.
• [9] Pilarek M., Neubauer P., Marx U.: Biological cardio-micro-pumps for microbioreactors and analytical micro-systems. Sensors and Actuators B: Chemical 156 (2011) 517-526.
• [10] Lowe K.C.: Perfluorochemical respiratory gas carriers: benefits to cell culture systems. Journal of Fluorine Chemistry 118 (2002) 19-26.
• [11] Pilarek M., Szewczyk K.W.: Perfluorochemicals as liquid vectors of respiratory gases used in medicine and biotechnology. Biotechnologia (in Polish) 69 (2005) 125-150.
• [12] Shiba Y., Ohshima T., Sato M.: Growth and morphology of anchorage-dependent animal cells in liquid/liquid interface system. Biotech. Bioeng. 57 (1998) 583-589.
• [13] Rappaport C.: Review - Progress in concept and practice of growing anchorage-dependent mammalian cells in three dimension. In Vitro Cellular and Developmental Biology -Animal 39 (2003) 187-192.
• [14] Pilarek M., Kaczmarczyk M., Olak S., Grabowska I., Szewczyk K.W.: Cultures of 3-D aggregated mammalian cells in a two-phase liquid/ liquid system containing perfluorinated carrier of respiratory gases. Acta Biochimica Polonica 58 Supplement 4 (2011) 5.

Uwagi

EN

This work was partially supported by MNT-ERA.NET Project "ArtiCart" financed by The National Centre for Research and Development (NCBiR; Poland).