Assessment of mutagenic activity of methyl- and phenylphenanthrenes based on Salmonella test and micronucleus test

• Autorzy: Rudnicka K. , Tejs S. , Budzikur K. A. , Mielżyńska-Švach D. , Jakimiuk E. , Chachaj A. , Góra M. , Żelazna K. , Łuczyński M. K.

Identyfikatory

DOI

10.14799/ebms193

• Języki publikacji: EN

Abstrakty

EN

Polycyclic aromatic hydrocarbons (PAHs) are widely spread environmental pollutants mainly originating from anthropogenic sources such as fossil fuel combustion, industries, and others. Although a large body of literature exists on the toxicity and carcinogenicity of PAHs, primarily benzo[a]pyrene, toxicity data for phenanthrene deriveratives are very limited. The main aim of the experiment was to investigate if there exists correlation between molecular structure and mutagenic activity of four phenanthrene derivatives: 1 methylphenanthrene, 4 methylphenanthrene, 1 phenylphenanthrene, and 4 phenylphenanthrene. An Ames assay using two strains of histidine dependent Salmonella Typhimurium (TA98 and TA100) was conducted to assess the mutagenic activity of studied compounds both in the presence (+S9) and in the absence (-S9) of an exogenous source of metabolic activation. The compounds were also tested in an in vitro chromosome aberration assay in which V-79 cells were exposed to the phenanthrene derivatives investigated both in the presence and in the absence of metabolic activation. The phenylphenanthrenes showed no mutagenic effect. These compounds occasionally induced significant decrease in the number of revertants in the Ames test. The greatest mutagenic effects were observed for 1 methylphenanthrene after metabolic activation (+S9). In the micronucleus test the greatest mutagenic effect was observed for 4 methylphenanthrene also in the presence of metabolic activation system. The results obtained are comparable to those reported earlier for the methylphenanthrenes.

Słowa kluczowe

EN

Ames test; in vitro; micronucleus test; mutagenicity; phenanthrene

PL

test Amesa; mutagenność; test mikrojądrowy; fenantren; in vitro

• Wydawca: University of Warmia and Mazury in Olsztyn, Poland
• Czasopismo: Environmental Biotechnology
• Rocznik: 2013
• Tom: Vol. 9, No. 2
• Strony: 65--71
• Opis fizyczny: Bibliogr. 38 poz., rys.

Twórcy

autor

Rudnicka K.

• Department of Environmental Biotechnology, University of Warmia and Mazury in Olsztyn, Sloneczna 45G, 10-718 Olsztyn, Poland; Phone: [phone]

autor

Tejs S.

• Department of Environmental Biotechnology, University of Warmia and Mazury in Olsztyn, Sloneczna 45G, 10-718 Olsztyn, Poland

autor

Budzikur K. A.

• Department of Environmental Biotechnology, University of Warmia and Mazury in Olsztyn, Sloneczna 45G, 10-718 Olsztyn, Poland

autor

Mielżyńska-Švach D.

• Institute of Occupational Medicine and Environmental Health, Kościelna 13, 41-200 Sosnowiec, Poland

autor

Jakimiuk E.

• Department of Veterinary Prevention and Feed Hygiene, University of Warmia and Mazury in Olsztyn, Oczapowskiego 14, 10-817 Olsztyn, Poland

autor

Chachaj A.

• Department of Organic Chemistry, Jagiellonian University, Ingardena 3, 30-060 Kraków, Poland

autor

Góra M.

• Department of Organic Chemistry, Jagiellonian University, Ingardena 3, 30-060 Kraków, Poland

autor

Żelazna K.

• Department of Environmental Biotechnology, University of Warmia and Mazury in Olsztyn, Sloneczna 45G, 10-718 Olsztyn, Poland

autor

Łuczyński M. K.

• Department of Chemistry, University of Warmia and Mazury in Olsztyn, Plac Łódzki 4, 10-957 Olsztyn, Poland

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