Nichrome Capacitors on Polycarbonate Substrate for Monitoring Cell Culture Using Impedance Sensing Technique

• Autorzy: Kociubiński A. , Zarzeczny D. , Prendecka M. , Pigoń D. , Małecka-Massalska T.

Identyfikatory

DOI

10.24425/amm.2020.131752

• Języki publikacji: EN

Abstrakty

EN

The aim of this work was to present a method of tissue culture research by measuring the impedance of cells cultured in the presence of nichrome. For this purpose, the Electric Cell-substrate Impedance Sensing system was used with a prototype substrate containing comb capacitors made of nichrome. Magnetron sputtering, photolithography and etching processes were used to produce the thin-film electrodes. In the experimental part, cells of mouse fibroblast cell line L929 were cultured according to the instruction manual in complete medium, under controlled growth conditions. Inoculation of arrays was carried out by 300 microliters per well of cell suspension at ~1.2×10⁵ cells/ml. The results of the monitoring cells behavior in tissue culture indicate good cell viability and proliferative potential.

Słowa kluczowe

EN

bioimpedance; ecis; sputtering; nichrome; fibroblast

• Wydawca: Institute of Metallurgy and Materials Science of Polish Academy of Sciences ; Committee of Materials Engineering and Metallurgy of Polish Academy of Sciences
• Czasopismo: Archives of Metallurgy and Materials
• Rocznik: 2020
• Tom: Vol. 65, iss. 1
• Strony: 493--496
• Opis fizyczny: Bibliogr. 11 poz., fot., rys.

Twórcy

autor

Kociubiński A.

• Lublin University of Technology, Faculty of Electrical Engineering and Computer Science, 38A Nadbystrzycka Str., 20-618 Lublin, Poland

autor

Zarzeczny D.

• Lublin University of Technology, Faculty of Electrical Engineering and Computer Science, 38A Nadbystrzycka Str., 20-618 Lublin, Poland

autor

Prendecka M.

• Medical University of Lublin, Faculty of Medicine, 11 Radziwiłłowska Str., 20-080 Lublin, Poland

autor

Pigoń D.

• Medical University of Lublin, Faculty of Medicine, 11 Radziwiłłowska Str., 20-080 Lublin, Poland

autor

Małecka-Massalska T.

• Medical University of Lublin, Faculty of Medicine, 11 Radziwiłłowska Str., 20-080 Lublin, Poland

Bibliografia

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Uwagi

EN

1. The publication was financed by the Ministry of Science and Higher Education under project 844/P-DUN/2019 zad. 2

PL

2. Opracowanie rekordu ze środków MNiSW, umowa Nr 461252 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2020).