Impact of membrane pore structure on protein detection sensitivity of affinity-based immunoassay

• Autorzy: Ahmad A. L. , Ideris N. , Ooi B.S. , Low S.C. , Ismail A.

Identyfikatory

DOI

10.1515/pjct-2016-0035

• Języki publikacji: EN

Abstrakty

EN

Understanding a membrane’s morphology is important for controlling its final performance during protein immobilization. Porous, symmetric membranes were prepared from a polyvinylidene fluoride/N-methyl-2-pyrrolidinone solution by phase inversion process, to obtain membrane with various microsized pores. The concentration and surface area of aprotein dotted on the membrane surface were measured by staining with Ponceau S dye. The dotted protein was further scanned and analysed to perform quantitative measurements for relative comparison. The intensity of the red protein spot and its surface area varied depending on the membrane pore size, demonstrating the dependence of protein immobilization on this factor. The membrane with the smallest pore size (M3) showed the highest protein spot intensity and surface area when examined at different protein concentrations. An increase in the applied protein volume showed a linearity proportional trend to the total surface area, and an uneven round dot shape was observed at a large applied volume of protein solution.

Słowa kluczowe

EN

morphology; PVDF membrane; protein immobilization

• Wydawca: West Pomeranian University of Technology. Publishing House
• Czasopismo: Polish Journal of Chemical Technology
• Rocznik: 2016
• Tom: Vol. 18, nr 2
• Strony: 97--103
• Opis fizyczny: Bibliogr. 29 poz., rys., tab.

Twórcy

autor

Ahmad A. L.

• Universiti Sains Malaysia, School of Chemical Engineering, Engineering Campus, Seri Ampangan, 14300 Nibong Tebal, S.P.S, Penang, Malaysia

autor

Ideris N.

• Universiti Teknologi MARA, Faculty of Chemical Engineering, 40450 Shah Alam, Selangor, Malaysia

autor

Ooi B.S.

• Universiti Sains Malaysia, School of Chemical Engineering, Engineering Campus, Seri Ampangan, 14300 Nibong Tebal, S.P.S, Penang, Malaysia

autor

Low S.C.

• Universiti Sains Malaysia, School of Chemical Engineering, Engineering Campus, Seri Ampangan, 14300 Nibong Tebal, S.P.S, Penang, Malaysia

autor

Ismail A.

• Universiti Sains Malaysia, Institute for Research in Molecular Medicine, Health Campus, 16150 Kubang Kerian, Kelantan, Malaysia

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Uwagi

Opracowanie ze środków MNiSW w ramach umowy 812/P-DUN/2016 na działalność upowszechniającą naukę.