Application of an aptamer and a reagent based on gold nanoparticles for detection of Escherichia coli

• Autorzy: Pięta P. P. , Szczerba A. , Dobroczyńska J. I. , Grabas K.

Identyfikatory

DOI

10.5277/epe130411

• Języki publikacji: EN

Abstrakty

EN

Plates functionalized with an Escherichia coll binding aptamer and a reagent based on gold nanoparticles were used for the detection of bacterial cells. Bacteria suspended in samples were caught by specific aptamers immobilized on a plastic plate whereas the gold nanoparticle reagent is an indicator reagent for visualization of the test result. Changes in colour of the gold nanoparticle reagent caused by gold nanoparticle agglomeration were observed with the naked eye. The test was designed for the detection of Escherichia coli in water suspensions.

Słowa kluczowe

EN

Escherichia coli; gold; aptamers; bacterial cells; gold nanoparticles; water suspensions

PL

Escherichia coli; złoto; aptamery; komórki bakteryjne; nanocząstki złota; zawiesiny wodne

• Wydawca: Oficyna Wydawnicza Politechniki Wrocławskiej
• Czasopismo: Environment Protection Engineering
• Rocznik: 2013
• Tom: Vol. 39, nr 4
• Strony: 125--133
• Opis fizyczny: Bibliogr. 11 poz., tab., rys.

Twórcy

autor

Pięta P. P.

• Department of Environmental Engineering, Wroclaw University of Technology, Wroclaw, Poland
• Central Laboratory of Medical Biology, Poznan University of Medical Sciences, Poznan, Poland

autor

Szczerba A.

• Department of Cell Biology, Poznan University of Medical Sciences, Poznan, Poland

autor

Dobroczyńska J. I.

• Department of Environmental Engineering, Wroclaw University of Technology, Wroclaw, Poland

autor

Grabas K.

• Department of Environmental Engineering, Wroclaw University of Technology, Wroclaw, Poland

Bibliografia

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