Rapid comparison of antitumor chemical constituents and mechanisms between Dendrobium nobile and Dendrobium officinale by UPLC-IT-TOF, network pharmacology and experimental verification

• Autorzy: Liu Jie , Li Zhaoyang , Zeng Meiling , Liang Yan , Liang Bing , Ge Qiuping , Zhang Xiaoyan , Zhang Shilin , Zhou Wei

Identyfikatory

DOI

10.1556/1326.2022.01012

• Języki publikacji: EN

Abstrakty

EN

Dendrobium nobile and Dendrobium officinale as the main varieties of traditional Chinese medicine Dendrobium are widely used in clinic. The study aimed to systematically explore chemical constituents and their antitumor effect of D. nobile and D. officinale by ultra-performance liquid chromatography coupled with ion trap time-of-flight mass spectrometry (UPLC-IT-TOF), network pharmacology and cancer cell experiments. D. nobile extract and D. officinale extract could significantly inhibit the proliferation of human lung cancer A549 cells, human liver cancer HepG2 cells and human breast cancer MCF-7 cells in the dose-dependent manner (P < 0.05), the antitumor effect of D. officinale extract was stronger than that of D. nobile extract at the same drug concentration. A total of 40 chemical constituents of D. nobile and D. officinale including phenanthrenes, bibenzyls and other types of compounds had been identified by UPLC-IT-TOF, LCMSsolution and MetID software according to retention times, accurate mass, MSⁿ fragmentation, reference compounds and natural product databases. Phenanthrenes with good antitumor activity were mainly present in D. nobile, bibenzyls were the main compounds of D. officinale. Integrated networks of Herb-Compounds-Targets-Cancer revealed that gigantol, moscatilin, tristin, moscatin and densiflorol B were regarded as key antitumor compounds of D. nobile and D. officinale, D. nobile and D. officinale shared 7 targets accounting for 70% of the antitumor core targets, more than half of their antitumor KEGG pathways were similar. The results of molecular docking and western blotting experiments indicated that the antitumor mechanisms of D. nobile and D. officinale may be through inhibiting PI3K-Akt and HIF-1α signaling pathways.

Słowa kluczowe

EN

antitumor; chemical constituent; Dendrobium nobile; Dendrobium officinale; natural medicine

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2023
• Tom: Vol. 35, no. 2
• Strony: 123--138
• Opis fizyczny: Bibliogr. 25 poz., tab., rys., wykr.

Twórcy

autor

Liu Jie

• Department of Pharmaceutical Analysis, School of Pharmacy, Guizhou Medical University, Guiyang 550025, Guizhou, PR China
• Department of Pharmacology, School of Basic Medicine, Guizhou Medical University, Guiyang 550025, Guizhou, PR China

autor

Li Zhaoyang

• Department of Pharmaceutical Analysis, School of Pharmacy, Guizhou Medical University, Guiyang 550025, Guizhou, PR China
• Department of Pharmacology, School of Basic Medicine, Guizhou Medical University, Guiyang 550025, Guizhou, PR China

autor

Zeng Meiling

• Department of Pharmaceutical Analysis, School of Pharmacy, Guizhou Medical University, Guiyang 550025, Guizhou, PR China
• Department of Pharmacology, School of Basic Medicine, Guizhou Medical University, Guiyang 550025, Guizhou, PR China

autor

Liang Yan

• Department of Pharmaceutical Analysis, School of Pharmacy, Guizhou Medical University, Guiyang 550025, Guizhou, PR China

autor

Liang Bing

• Department of Pharmacology, School of Basic Medicine, Guizhou Medical University, Guiyang 550025, Guizhou, PR China

autor

Ge Qiuping

• Center for Drug Research and Innovation, Guiyang Dechangxiang Pharmaceutical Co., Ltd., Guiyang 550201, Guizhou, PR China

autor

Zhang Xiaoyan

• Department of Pharmacology, School of Basic Medicine, Guizhou Medical University, Guiyang 550025, Guizhou, PR China

• https://orcid.org/0000-0002-1597-3038

autor

Zhang Shilin

• Center for Drug Research and Innovation, Guiyang Dechangxiang Pharmaceutical Co., Ltd., Guiyang 550201, Guizhou, PR China

• https://orcid.org/0000-0002-1295-8641

autor

Zhou Wei

• Department of Pharmaceutical Analysis, School of Pharmacy, Guizhou Medical University, Guiyang 550025, Guizhou, PR China

• https://orcid.org/0000-0002-4123-2276

Bibliografia

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Uwagi

Opracowanie rekordu ze środków MEiN, umowa nr SONP/SP/546092/2022 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2022-2023).