Vascular smooth muscle cells in cultures on low density polyethylene modified with plasma discharge and biofunctionalization

• Autorzy: Parizek M. , Kasalkova N. , Bacakova L. , Kolarova K. , Lisa V. , Svorcik V.
• Języki publikacji: EN

Abstrakty

EN

Low density polyethylene (LDPE) was modified by an Ar plasma discharge and then grafted with glycine (Gly), bovine serum albumin (BSA) or polyethylene glykol (PEG). Some plasma-treated samples and samples grafted with BSA were exposed to a suspension of colloidal carbon particles (C, BSA+C). Pristine LDPE and tissue culture polystyrene dishes (PSC) were used as control samples. The materials were seeded with rat aortic smooth muscle cells and incubated in a medium DMEM with 10% of fetal bovine serum. On day 1 after seeding, the cells on LDPE modified with plasma only, Gly, BSA and BSA+C adhered in similar numbers as on PSC, while the values on non-modified and PEG-modified samples were significantly lower. On day 5, the highest cell numbers were found again on LDPE with Gly, BSA and BSA+C. On day 7, the highest number of cells was found on LDPE modified only with plasma. The latter cells also dis-played the largest cell spreading area. The increased cell colonization was probably due to the formation of oxygen-containing chemical functional groups after plasma irradiation, and also due to positive effects of grafted Gly, BSA and BSA in combination with colloidal C particles.

Słowa kluczowe

EN

Ar plasma discharge; biomaterials; low-density polyethylene; cell adhesion; cell proliferation; grafting; tissue engineering; vascular smooth muscle cells

• Wydawca: Polish Society for Biomaterials in Cracow
• Czasopismo: Engineering of Biomaterials
• Rocznik: 2009
• Tom: Vol. 12, no. 89-91
• Strony: 25--28
• Opis fizyczny: Bibliogr. 13 poz., wykr., zdj.

Twórcy

autor

Parizek M.

• Institute of Physiology, Acad. Sci. Cr, Videnska 1083, 142 20 Prague4-Krc, Czech Republic

autor

Kasalkova N.

• Institute of Chemical Technology, Technicka 5, 166 28 Prague 6 – Dejvice

autor

Bacakova L.

• Institute of Physiology, Acad. Sci. Cr, Videnska 1083, 142 20 Prague4-Krc, Czech Republic

autor

Kolarova K.

• Institute of Chemical Technology, Technicka 5, 166 28 Prague 6 – Dejvice

autor

Lisa V.

• Institute of Physiology, Acad. Sci. Cr, Videnska 1083, 142 20 Prague4-Krc, Czech Republic

autor

Svorcik V.

• Institute of Chemical Technology, Technicka 5, 166 28 Prague 6 – Dejvice

Bibliografia

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