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Abstrakty
Low density polyethylene (LDPE) was modified by an Ar plasma discharge and then grafted with glycine (Gly), bovine serum albumin (BSA) or polyethylene glykol (PEG). Some plasma-treated samples and samples grafted with BSA were exposed to a suspension of colloidal carbon particles (C, BSA+C). Pristine LDPE and tissue culture polystyrene dishes (PSC) were used as control samples. The materials were seeded with rat aortic smooth muscle cells and incubated in a medium DMEM with 10% of fetal bovine serum. On day 1 after seeding, the cells on LDPE modified with plasma only, Gly, BSA and BSA+C adhered in similar numbers as on PSC, while the values on non-modified and PEG-modified samples were significantly lower. On day 5, the highest cell numbers were found again on LDPE with Gly, BSA and BSA+C. On day 7, the highest number of cells was found on LDPE modified only with plasma. The latter cells also dis-played the largest cell spreading area. The increased cell colonization was probably due to the formation of oxygen-containing chemical functional groups after plasma irradiation, and also due to positive effects of grafted Gly, BSA and BSA in combination with colloidal C particles.
Czasopismo
Rocznik
Strony
25--28
Opis fizyczny
Bibliogr. 13 poz., wykr., zdj.
Twórcy
autor
- Institute of Physiology, Acad. Sci. Cr, Videnska 1083, 142 20 Prague4-Krc, Czech Republic
autor
- Institute of Chemical Technology, Technicka 5, 166 28 Prague 6 – Dejvice
autor
- Institute of Physiology, Acad. Sci. Cr, Videnska 1083, 142 20 Prague4-Krc, Czech Republic
autor
- Institute of Chemical Technology, Technicka 5, 166 28 Prague 6 – Dejvice
autor
- Institute of Physiology, Acad. Sci. Cr, Videnska 1083, 142 20 Prague4-Krc, Czech Republic
autor
- Institute of Chemical Technology, Technicka 5, 166 28 Prague 6 – Dejvice
Bibliografia
- [1]. Bacakova L., Svorcik V., Rybka V., Micek I., Hnatowitz V., Lisa V., Kocourek F.: Biomaterials 17: 1121-1126, 1996.
- [2]. Bacakova L., Mares V., Bottone M.G., Pellicciari C., Lisa V., Svorcik V.: J. Biomed. Mater. Res. 49: 369-379, 2000.
- [3]. Bacakova L., Walachova K., Svorcik V., Hnatowitz V.: J. Biomater. Sci. Polymer Edn., 12: 817-834, 2001.
- [4]. Svorcik V., Rockova K., Ratajova E., Heitz J., Huber N., Bäuerle D., Bacakova L., Dvorankova B., Hnatowitz V.: Nucl. Instr. Meth. Phys. Res. B 217: 307-313, 2004.
- [5]. Turos A., Jagielski J., Piatkowska A., Bielinski D., Slusarski L., Madi N.K.: Vacuum, 70: 201-206, 2003.
- [6]. Wang Y., Lu L., Zhang Y., Chen X.: J. Biomed. Mater. Res. A 76: 589-595, 2006.
- [7]. Kasalkova N., Kolarova K., Bacakova L., Parizek M., Svorcik V.: Cell adhesion and proliferation on modified PE. Mater. Sci. Forum 567-568: 269-272, 2007.
- [8]. Van Amerongen A., Wichers J.H., Berendsen L.B.J.M., Timmermans A.J.M., Keizer G.D., Van Doorn A.W.J., Bantjes A., Van Gelder W.M.J.: J. Biotechnol. 30: 185-195, 1993.
- [9]. Bacakova L., Filova E., Kubies D., Machova L., Proks V., Malinova V., Lisa V., Rypacek F.: J. Mater. Sci. Mater. Med. 18: 1317-1323, 2007.
- [10]. Grinevich A., Bacakova L., Choukourov A., Boldyryeva H., Pihosh Y., Slavinska D., Noskova L., Skuciova M., Lisa V., Biederman H.: J. Biomed. Mater. Res. 88A: 952-966, 2009.
- [11]. Koblinski J.E., Wu M., Demeler B., Jacob K., Kleinman H.K.: Matrix cell adhesion activation by non-adhesion proteins. J. Cell. Sci. 118: 2965-2974, 2005.
- [12]. Liu L., Chen S., Giachelli C. M., Ratner B. D., Jiang S.: J. Biomed. Mater. Res. A 74: 23-31, 2005.
- [13]. Svorcik V., Kolarova K., Slepicka P., Mackova A., Novotna M., Hnatowicz V.: Polym. Degr. Stab.91: 1219-1 225, 2006.
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-6f6c6dbe-6183-40c0-aa37-0fafa1d2f014