Production of (R)-styrene oxide by recombinant whole-cell biocatalyst in aqueous and biphasic system

• Autorzy: Xue F. , Gao J.

Identyfikatory

DOI

10.2478/pjct-2018-0023

• Języki publikacji: EN

Abstrakty

EN

The enantioselective resolution of racemic styrene oxide (rac-SO) to (R)-SO by whole cells of a recombinant Escherichia coli expressing epoxide hydrolase (EH) activity in aqueous and biphasic system were studied. Some parameters that may alter this bio-resolution, such as the concentration of recombinant cell, substrate and product were evaluated. The effect of the addition of different additives on the course of rac-SO biotransformation was also investigated. The results showed that the yield and the enantiomeric excess (ee) of (R)-SO were dependent on these variables. When the kinetic resolution was conducted with 350 mM of rac-SO, enantiopure (R)-SO with high (≥99%) ee was obtained with a yield of 38.2% yield at 12.2 h in the presence of 10% (v/v) Tween 80. An isooctane/aqueous system was developed to overcome the adverse factors in the aqueous phase, resulting in an improvement of yield from 38.2% to 42.9%. The results will provide a useful guidance for further application of this enzyme in the biocatalytic production of chiral synthons.

Słowa kluczowe

EN

Kinetic resolution; Epoxide hydrolase; Enantioselectivity; (R)-styrene oxide

• Wydawca: West Pomeranian University of Technology. Publishing House
• Czasopismo: Polish Journal of Chemical Technology
• Rocznik: 2018
• Tom: Vol. 20, nr 2
• Strony: 54--60
• Opis fizyczny: Bibliogr. 39 poz., rys., tab.

Twórcy

autor

Xue F.

• School of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng 224051, P.R. China

autor

Gao J.

• School of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng 224051, P.R. China

Bibliografia

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Uwagi

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2018).