Biotypowanie mikroorganizmów za pomocą spektrometrii mas oraz spektroskopii NMR

• Autorzy: Mielko Karolina Anna , Młynarz Piotr

Warianty tytułu

EN

Biotyping of microorganisms using mass spectrometry and NMR spectroscopy

• Języki publikacji: PL

Abstrakty

EN

Typing microorganisms is a very important element of laboratory diagnostics. Appropriate recognition of the pathogen and determination of its sensitivity to the drugs used is necessary to start treatment. There are many types of microbial typing. The most popular division is genotypic and phenotypic typing (among which biotyping, antibiotic resistance analysis, and protein profile analysis are the most common) or phagotyping) [1, 2]. Recently, there has been a very rapid development of mass spectrometry techniques as a method for identifying microorganisms [3]. Mass spectrometry (MS) and nuclear magnetic resonance spectroscopy (NMR) are methods that allow the comparison of metabolomic profiles of microorganisms. These are also methods commonly used in metabolomics. Metabolomics is a field of science dealing with the analysis of low-mass compounds characteristic of the studied material [4]. Therefore, the use of metabolomics in microbiology allows to identify and discriminate of microorganisms [5]. Recently, the analyzes also apply to metabolites. Many studies focus on the analysis of volatile organic compounds (VOCs) that allow the analysis of samples directly from the patient without the need for isolation of a single microorganism [6, 7]. Recent studies show many possibilities for the use of NMR spectroscopy. The results of the analysis show that it is also a method that allows the identification and differentiation of strains of microorganisms. Thanks to this method it is also possible to determine the origin of the strain or to indicate its resistance to antibiotics. [10, 11]. Improvement of research algorithms used in metabolomics for biotyping microorganisms may in the future allow for the creation of a fast, accurate and cheap way to identify microorganisms. Proteomic tests using the MS method are very popular, in which protein profiles of strains are analyzed and compared. These studies are mainly conducted using MALDI-TOF MS mass spectrometry. This technique is now quite widely used in microbiological diagnostics [10]. The research confirms the high discrimination power of this method [11].

Słowa kluczowe

PL

mikrobiologia; biotypowanie; NMR; MS; metabolomika

EN

biotyping; NMR; MS; metabolomics

• Wydawca: Polskie Towarzystwo Chemiczne
• Czasopismo: Wiadomości Chemiczne
• Rocznik: 2020
• Tom: [Z] 74, 1-2
• Strony: 57--70
• Opis fizyczny: Bibliogr. 48 poz., rys.

Twórcy

autor

Mielko Karolina Anna

• Katedra Biochemii, Biologii Molekularnej i Biotechnologii, Wydział Chemiczny, Politechnika Wrocławska, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław

autor

Młynarz Piotr

• Katedra Biochemii, Biologii Molekularnej i Biotechnologii, Wydział Chemiczny, Politechnika Wrocławska, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław

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Uwagi

Opracowanie rekordu ze środków MNiSW, umowa Nr 461252 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2020).