Determination of amoxicillin in poultry plasma by high-performance liquid chromatography after formaldehyde derivation

• Autorzy: Krasucka D , Kowalski C. J , Osypiuk M , Opielak G
• Języki publikacji: EN

Abstrakty

EN

A high-performance liquid chromatographic (HPLC) method with fluorescence detection after precolumn formaldehyde derivation was developed to detect concentrations of amoxicillin (AMX) in poultry plasma. Proteins in plasma samples spiked with AMX were precipitated with a phosphate buffer and trichloroacetic acid. After precolumn treatment of the extraction product of AMX with formaldehyde under acidic and heating conditions, HPLC analysis with fluorescence (FL) detection at an excitation wavelength of 355 nm and an emission wavelength of 450 nm was performed. A mobile phase comprising acetonitrile and a buffer solution (0.05 M KH2PO4 pH = 5.6), which yielded AMX retention time 8.58 min, was suitable for detection of AMX. The calculated standard curve of the reaction product was linear, and the correlation coefficient was greater than 0.999. The limit of detection and quantification, the accuracy, and the precision were evaluated. Recoveries of spiked amoxicillin were >92%, with a coefficient of variation in the range of 0.35–0.89%. This method has been successfully applied to a pharmacokinetic study after oral administration of amoxicillin to poultry.

Słowa kluczowe

EN

amoxicillin; HPLC; validation; poultry

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2015
• Tom: Vol. 27, no. 1
• Strony: 55--65
• Opis fizyczny: Bibliogr. 23 poz., rys., tab.

Twórcy

autor

Krasucka D

• National Veterinary Research Institute — National Research Institute Department of Veterinary Pharmacy Al. Partyzantów 57 24-100 Pulawy Poland

autor

Kowalski C. J

• University of Life Sciences in Lublin Department of Pharmacology, Faculty of Veterinary Medicine Akademicka 12 20-033 Lublin Poland

autor

Osypiuk M

• University of Life Sciences in Lublin Department of Pharmacology, Faculty of Veterinary Medicine Akademicka 12 20-033 Lublin Poland

autor

Opielak G

• Medical University School of Lublin Human Anatomy Department Jaczewskiego 4 20-090 Lublin Poland

Bibliografia

• [1] S.A. Carlson and K.E. Ferris, Vet. Microbiol., 73, 25 (2000)
• [2] Ch.B.V.N. Raju, H.K. Sharma, Ch.S. Rao, and G.N. Rao, Acta Chromatogr., 21, 57 (2009)
• [3] D.G.S. Burch, Bvetmed MRCVS Antimicrobial Sensitivity Data for Major Pig Bacterial Pathogens, Octagon Services Ltd. Copyright, January 2002 A, www.octagonservices. co.uk
• [4] M. Czerwińska-Szymańska, K. Pietruszka, and D. Bednarek, Bull. Vet. Inst. Pulawy, 51, 393 (2007)
• [5] C. Hong and F. Kondo, J. Food Prot., 63, 1421 (2000)
• [6] N. Furusawa, Fresenius J. Anal. Chem., 368, 624 (2000)
• [7] C.Y. Ang, W. Luo, E.B. Jr. Hansen, J.P. Freeman, and H.C. Jr. Thompson, J. — Assoc. Off. Anal. Chem., 79, 389 (1996)
• [8] W. Luo and C.Y. Ang, J. — Assoc. Off. Anal. Chem., 81, 20 (2000)
• [9] W. Luo, K. Ang, and H.C. Jr. Thomso, J. Chromatogr., B: Biomed. Sci. Appl., 694, 401 (1997)
• [10] M. Dousa and R. Hosmanová, J. Pharm. Biomed. Anal., 37, 373 (2005)
• [11] S. De Baere, P. De Backer, Anal. Chim. Acta, 586, 319 (2007)
• [12] Q. Pei, G.P. Yang, Z.J. Li, X.D. Peng, J.H. Fan, and Z.Q. Liu, J. Chromatogr., B: Anal. Technol. Biomed. Life Sci., 879, 2000 (2011)
• [13] W. Luo, E.B. Jr. Hansen, C.Y. Ang, J. Deck, J.P. Freeman, and H.C. Jr. Thompson, J. Agric. Food Chem., 45, 1264 (1997)
• [14] R. Fernandez-Torres, M.O. Consentino, M.A. Lopez, and M.C. Mochon, Talanta, 81, 871 (2010)
• [15] W. Li, F. Tan, and K. Zhao, J. Pharm. Biomed. Anal., 41, 594 (2006)
• [16] X. Du., C. Li., H.K. Sun, C.H. Nightingale, and D.P. Nicolau, J. Pharm. Biomed. Anal., 39, 648 (2005)
• [17] V.F. Samanidou, D.E. Giannakis, and A. Papadaki, J. Sep. Sci., 32, 1302 (2009)
• [18] C.A.I. Jin-hua, G.U. Xin, and L.I.U. Ya-ni, Chinese J. Vet. Drug, 7, 6 (2005)
• [19] C.M. Stobba-Wiley and R.S. Readnour, J. AOAC Int., 83 (3), 555 (2000)
• [20] M.S. Berenstein and M.A. Evans, J. Chromatogr., 229, 179 (1982)
• [21] F.T. Peters, O.H. Drummer, and F. Musshoff, Forensic Sci. Int., 165, 216 (2007)
• [22] W. Sripinyowanich, S. Wentzel, and A. Nuntaprasert, Proceedings of the 5th Asian Pig Veterinary Society Congress 7–9 March 2011, Pattaya, Thailand
• [23] D. Krasucka and C.J. Kowalski, Acta Pol. Pharm., 67, 729 (2010)