Microfluidic devices — application in anticancer studies

• Autorzy: Jędrych E. , Chudy M. , Dybko A. , Brzózka Z.
• Języki publikacji: EN

Abstrakty

EN

A rapidly growing pharmaceutical industry requires faster and more efficient ways to find and test new drugs. One of the new method for cell culture and examining the toxic effects of drugs is application of microfluidic systems. They provide new types of microenvironments and new methods for investigation of anticancer therapy. The use of microsystems is a solution that gives the opportunity to reduce not only cost and time, but also a number of tests on animals. In this paper we present designed and fabricated hybrid microfluidic systems which are applicable for cell culture, cell based cytotoxicity assays and photodynamic therapy procedures. Polydimethylsiloxane (PDMS) and sodium glass were used for fabrication of microdevices. The designed geometry of the microdevices includes cell culture microchambers and a concentration gradient generator (CGG). The CGG enables to obtain diff erent concentrations of tested drugs in a single step, which is a significant simplification of cytotoxicity assay procedure. In the designed microsystems three various cell lines (normal and carcinoma) were cultured and analyzed.

Słowa kluczowe

EN

microfluidic system; PDMS; adherent cell culture; concentration gradient generator (CGG); cytotoxicity tests; photodynamic therapy (PDT) procedures

• Wydawca: Foundation for Young Scientists
• Czasopismo: Challenges of Modern Technology
• Rocznik: 2012
• Tom: Vol. 3, no. 2
• Strony: 3--5
• Opis fizyczny: Bibliogr. 5 poz., tab., rys.

Twórcy

autor

Jędrych E.

• Department of Microbioanalytics, Faculty of Chemistry, Warsaw University of Technology, Noakowskiego 3, 00-664 Warsaw

autor

Chudy M.

autor

Dybko A.

autor

Brzózka Z.

Bibliografia

• [1] Labianca, R, S. Moscon i, and M.C. Garassino. Ann. Oncol. 17 (2006): 51–54.
• [2] Shah, M.A., and G.K. Schwartz. Clinical Cancer Research 7 (2001): 2168–2181.
• [3] Betz, C.S., et al. Photochem Photobiol Sci. 1 (2002): 15--19.
• [4] Manivasager, V., et al. Int J Oncol. 29 (2006): 997--1002.
• [5] Dodge, A., et al. “PDMS-based microfl uidics for proteomic analysis”. Analyst 131 (2006): 1122–1128.