Spiral-coil countercurrent chromatographic separation of betanin and betanidin ethyl-esters monitored by continuous ESI-MS/MS injection and LC-ESI-MS/MS

• Autorzy: Tuwalska D. , Szot D. , Wybraniec S. , Winterhalter P. , Jerz G.
• Języki publikacji: EN

Abstrakty

EN

Betalains present in Beta vulgaris L. (Chenopodiaceae) have a high potential as natural pigments for food applications. They have been used as a substitute of synthetic dyes in processing of gelatine, strawberry yogurts, ice creams, fruits cocktails, candies and biscuits. The stability of betalains is strongly influenced by numerous factors – sugar content, and impact of light, oxygen, water activity, pH and temperature. A mixture of betacyanin mono-, di- and tri-ethyl-esters was separated by a large-volume spiral-coil countercurrent chromatography (spCCC) prototype which yielded fractions continuously off-line injected to an ESI-MS/MS device. This method yielded a reconstituted pigment profile and finally enabled the MS-target-guided isolation procedure for the whole spCCC-experiment. The individual fractions were analyzed by LC/ESI-MS/MS. Betanin and betanidin mono-, di- and tri-ethyl-esters as well as other decarboxylated derivatives were detected in the chromatograms. The ethyl-esters of betanin were only partially fractionated, but clearly fractionated from the ethyl-esters of betanidin which will be of great value for biological evaluations.

Słowa kluczowe

EN

spiral-coil countercurrent chromatography (spCCC); betacyanin ethyl-esters; betalains; betacyanins; betanin; betanidin

• Wydawca: Foundation for Young Scientists
• Czasopismo: Challenges of Modern Technology
• Rocznik: 2013
• Tom: Vol. 4, no. 4
• Strony: 21--25
• Opis fizyczny: Bibliogr. 10 poz., rys., wykr., tab.

Twórcy

autor

Tuwalska D.

• Department of Analytical Chemistry, Institute C-1, Faculty of Chemical Engineering and Technology, Cracow University of Technology, ul. Warszawska 24, Cracow 31-155, Poland, phone: [phone]

autor

Szot D.

• Department of Analytical Chemistry, Institute C-1, Faculty of Chemical Engineering and Technology, Cracow University of Technology, ul. Warszawska 24, Cracow 31-155, Poland, phone: [phone]

autor

Wybraniec S.

• Department of Analytical Chemistry, Institute C-1, Faculty of Chemical Engineering and Technology, Cracow University of Technology, ul. Warszawska 24, Cracow 31-155, Poland, phone: [phone]

autor

Winterhalter P.

• Institute of Food Chemistry, Technische Universität Braunschweig, Braunschweig, Germany

autor

Jerz G.

• Institute of Food Chemistry, Technische Universität Braunschweig, Braunschweig, Germany

Bibliografia

• [1] Bilyk A. “Extractive fractionation of betalains”. Journal of Food Science 44 (1979): 1249-1251.
• [2] Moreno-Alvarez M.J., Viloria-Matos A., and D.M. Hidalgo-Báez “A new method for the isolation of betalaines by HPTLC”. Revista de la Facultad de Agronomía (LUZ) 21 (2004): 140-144.
• [3] Bilyk A. “Thin-Layer Chromatographic Separation of Beet Pigments”. Journal of Food Science 46 (1981): 298-299.
• [4] Delgado-Vargas F., Jiménez R., and O. Paredes-López. “Natural Pigments: Carotenoids, Anthocyanins, and Betalains - Characteristics, Biosynthesis, Processing, and Stability”. Critical Reviews in Food Science and Nutrition 40(3) (2000): 173-289.
• [5] Jerz G., Skotzki T., Fiege K., Winterhalter P., and S. Wybraniec. “Separation of betalains from berries of Phytolacca Americana by ion-pair high-speed counter-current chromatography”. Journal of Chromatography A 1190 (2008): 63-73.
• [6] Berthord A. et al. “Countercurrent chromatography in analytical chemistry”. Pure and Applied Chemistry 81 (2009): 355-387.
• [7] Cao X., Pei H., Huo L., Hu G., and Y. Ito. “Development and evaluation of a spiral tube column for counter-current chromatography”.Journal of Separation Science 34 (2011): 2611-2617
• [8] Wybraniec S, Stalica P., Jerz G., Klose B., Gebert N., Winterhalter P., Spórna A., Szaleniec M., and Y. Mizrahi. “Separation of polar betalain pigments from cacti fruits of Hylocereus polyrhizus by ion-pair-high-speed countercurrent chromatography”. Journal of Chromatography A 1216 (2009) 6890-6899.
• [9] Wybraniec S., Jerz G., Gebers N., and P. Winterhalter. “Ionpair high-speed countercurrent chromatography in fractionation of a high-molecular weight variation of acyl-oligosaccharide linked betacyanins from purple bracts of Bougainvillea glabra”.Journal of Chromatography B 878 (2010): 538-550.
• [10] Yao S., Cao Y., Jia Ch., Wang Y., Song H. “Developments of instruments and methods related with high-speed countercurrent chromatography and their applications in research of natural medicines”. Central European Journal of Chemistry 10(3) (2012): 417-432.