Evaluation of the biological impact of the mixtures of diclofenac with its biodegradation metabolites 4’-hydroxydiclofenac and 5-hydroxydiclofenac on Escherichia coli. DCF synergistic effect with caffeic acid

Matejczyk, Marzena; Ofman, Piotr; Dąbrowska, Katarzyna; Świsłocka, Renata; Lewandowski, Włodzimierz

doi:10.24425/aep.2020.135760

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Tytuł artykułu

Evaluation of the biological impact of the mixtures of diclofenac with its biodegradation metabolites 4’-hydroxydiclofenac and 5-hydroxydiclofenac on Escherichia coli. DCF synergistic effect with caffeic acid

Autorzy

Matejczyk Marzena , Ofman Piotr , Dąbrowska Katarzyna , Świsłocka Renata , Lewandowski Włodzimierz

Treść / Zawartość

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Identyfikatory

DOI

10.24425/aep.2020.135760

Warianty tytułu

Ocena oddziaływania diclofenaku i jego metabolitów biodegradacji 4'-hydroksydiklofenaku i 5-hydroksydiklofenaku na Escherichia coli. Synergistyczny efekt diklofenaku z kwasem kawowym

Języki publikacji

Abstrakty

In environmental matrices there are mixtures of parent drug and its metabolites. The majority of research is focused on the biological activity and toxic effect of diclofenac (DCF), there is little research on the biological activity of DCF metabolites and their mixtures. The study focused on the assessment of the biological impact of DCF, its metabolites 4’-hydroxydiclofenac (4’-OHDCF) and 5-hydroxydiclofenac (5-OHDCF) and their mixtures on E. coli strains. The biological effects of tested chemicals were evaluated using the following: E. coli K-12 cells viability assay, the inhibition of bacteria culture growth, ROS (reactive oxygene species) generation and glutathione (GSH) content estimation. Moreover, we examined the influence of the mixture of DCF with caffeic acid (CA) on E. coli cells viability. Our results showed the strongest impact of the mixtures of DCF with 4’-OHDCF and 5-OHDCF on E. coli SM biosensor strains in comparison to parent chemicals. Similar results were obtained in viability test, where we noticed the highest reduction in E. coli cell viability after bacteria incubation with the mixtures of DCF with 4’-OHDCF and 5-OHDCF. Similarly, these mixtures strongly inhibited the growth of E. coli culture. We also found synergistic effect of caffeic acid in combination with DCF on E. coli cells viability. After bacteria treatment with the mixture of DCF and its metabolites we also noted the strongest amount of ROS generation and GSH depletion in E. coli culture. It suggests that oxidative stress is the most important mechanism underlying the activity of DCF and its metabolites.

Celem pracy było określenie oddziaływania diklofenaku, jego metabolitów biodegradacji 4’-OHDCF i 5-OHDCF oraz ich mieszanin na szczepy E. coli. Efekt biologiczny i stres oksydacyjny wywołany działaniem badanych w pracy związków chemicznych oceniono, poddając analizie następujące biomarkery: żywotność komórek E. coli K-12, hamowanie wzrostu kultury bakterii, wytwarzanie ROS i ocena zawartości glutationu (GSH). Ponadto zbadaliśmy wpływ mieszaniny DCF z CA na żywotność komórek E. coli. Monitorowaliśmy także reaktywność szczepu biosensora E. coli SM recA: luxCDABE w ściekach. Otrzymane wyniki wykazały najsilniejszy wpływ mieszanin DCF z 4’-OHDCF i 5-OHDCF na szczepy E. coli. Mieszanki diclofenaku z metabolitami działały inhibująco na rozwój kultury E. coli K-12 i żywotność komórek. Zaobserwowano także synergistyczne, inhibitorowe działanie kwasu kawowego w połączeniu z DCF na żywotność komórek E. coli. Najintensywniejszą generację ROS oraz redukcję GSH zaobserwowano po potraktowaniu bakterii mieszaniną DCF i jej metabolitów. Sugeruje to, że stres oksydacyjny jest najważniejszym mechanizmem leżącym u podstaw działania DCF i jego metabolitów. Ponadto, w przeprowadzonym eksperymencie wykazano użyteczność mikrobiologicznego biosensora E. coli SM recA w monitorowaniu ścieków zanieczyszczonych DCF. Uzyskane wyniki wskazują, że metabolity DCF 4’-OHDCF i 5-OHDCF mają zdolność interakcji z DCF. Zaobserwowaliśmy, że mieszaniny DCF z metabolitami mają większy wpływ na żywotność i rozwój kultury E. coli oraz indukcję promotorów w biosensorowych szczepach E. coli.

Słowa kluczowe

diclofenac biodegradation metabolites wastewaters microbial biosensors

diklofenak biodegradacja metabolity ścieki bioczujniki mikrobiologiczne

Wydawca

Institute of Environmental Engineering, Polish Academy of Sciences

Czasopismo

Archives of Environmental Protection

Rocznik

2020

Tom

Vol. 46, no. 4

Strony

10--22

Opis fizyczny

Bibliogr. 55 poz., rys., wykr., tab.

Twórcy

autor

Matejczyk Marzena

m.matejczyk@pb.edu.pl

Bialystok University of Technology, Faculty of Civil Engineering and Environmental Sciences, Division of Chemistry, Biology and Biotechnology, Bialystok, Poland

autor

Ofman Piotr

Bialystok University of Technology, Faculty of Environmental Engineering Technology and Systems, Bialystok University of Technology, Bialystok, Poland

autor

Dąbrowska Katarzyna

Department of Microbiology, Institute of Agricultural and Food Biotechnology, Warsaw, Poland

autor

Świsłocka Renata

Bialystok University of Technology, Faculty of Civil Engineering and Environmental Sciences, Division of Chemistry, Biology and Biotechnology, Bialystok, Poland

autor

Lewandowski Włodzimierz

Bialystok University of Technology, Faculty of Civil Engineering and Environmental Sciences, Division of Chemistry, Biology and Biotechnology, Bialystok, Poland

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Uwagi

Opracowanie rekordu ze środków MNiSW, umowa Nr 461252 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2021).

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Bibliografia

Identyfikator YADDA

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