UPLC-DAD determination of taxodione content in hairy roots of Salvia austriaca Jacq.

• Autorzy: Kuźma Ł. , Wysokińska H.
• Języki publikacji: EN

Abstrakty

EN

Taxodione is the abietane diterpenoid, which has been earlier reported to show cytotoxic, antitumor, antibacterial, and antioxidant properties. This compound has been isolated from a genetically transformed root culture of Salvia austriaca. However, no method for taxodione quantification is yet available. A validated ultra-performance liquid chromatography-diode array detector (UPLC-DAD) method was developed for the quantification of taxodione in a hexane extract taken from genetically transformed roots of S. austriaca. The developed UPLC-DAD method demonstrated good linearity in the range 10–500 μg mL−1 (r2 = 0.99988), high precision (relative standard deviation (RSD) <1.42%), and good repeatability (RSD <1.84%). The limits of detection and quantification of taxodione were found to be 0.48 ng and 1.60 ng, respectively. The recovery of taxodione ranged between 90.4 and 113.1%, and the RSD was less than 2.5%.The developed UPLC-DAD method was then used to determine the content of taxodione in various hairy root lines and changes in amount of the compound during the 50-day growth cycle of the culture. The content of taxodione in hairy root cultures of S. austriaca ranged from 0.29 to 1.12 mg g−1 dry weight depending on root line and stage of growth.

Słowa kluczowe

EN

taxodione; Salvia austriaca hairy roots; ultra performance liquid chromatography

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2014
• Tom: Vol. 26, no. 4
• Strony: 671--681
• Opis fizyczny: Bibliogr. 8 poz., rys., tab.

Twórcy

autor

Kuźma Ł.

• Medical University of Łódź Department of Biology and Pharmaceutical Botany Muszyńskiego 1 st. 90-151 Łódź Poland

autor

Wysokińska H.

• Medical University of Łódź Department of Biology and Pharmaceutical Botany Muszyńskiego 1 st. 90-151 Łódź Poland

Bibliografia

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