Research on solvent systems with tetra-n-butylammonium bromide for counter-current chromatography of betalains

• Autorzy: Spórna-Kucab A. , Wysoczańska J. , Bartel A. , Świergosz T. , Szot D. , Wybraniec S.
• Języki publikacji: EN

Abstrakty

EN

In this study, new two-phase solvent systems for counter-current chromatography (CCC) consisting of n-butanol and water as well as various amounts of acetic acid, acetonitrile, ethanol, acetone or ethyl acetate were tested. Additionally, tetra-n-butylammonium bromide (TBAB) was introduced into the system in the form of aqueous solutions or phosphate-citrate buffer (pH 6.7) in order to form ion-pairs with betalains. The selection of buffer pH was based on their ability to create ion pairs by tetraalkylammonium salts, with selected betalains under these conditions. In this study, it is shown that the settling time of two phases is longer with the increase of acetic acid/acetonitrile/ethanol/acetone/ethyl acetate. For selected solvent systems with high amounts of acetonitrile, ethanol and acetone two phases were not observed. The systems with acetone have the largest increase of settling time. Ethyl acetate systems were characterized by a slow settling time increase. In systems containing additionally 2% aqueous TBAB, smaller changes in settling time than in similar systems without TBAB were observed. Addition of TBAB in the buffer resulted in a prolongation of settling time. Solvent systems in which the separation between the aqueous and organic phases was visually best, were selected from among all the tested systems and the betalain partition coefficients were measured by LC-DAD-ESI-MS. The best results were observed for systems: n-butanol-water-acetic acid (2:2.5:0.75, v/v/v), n-butanol-water-acetic acid (2:2.5:1, v/v/v) and n-butanol-TBAB in water-acetonitrile (2:2.5:0.5, v/v/v).

Słowa kluczowe

EN

betanin; betalains; tetra-n-butylammonium bromide; high-speed counter-current chromatography; Beta vulgaris L.

PL

betanina; betalainy; bromek tetra-n-butyloamoniowy; HSCCC; Beta vulgaris L.

• Wydawca: Foundation for Young Scientists
• Czasopismo: Challenges of Modern Technology
• Rocznik: 2016
• Tom: Vol. 7, no. 1
• Strony: 12--16
• Opis fizyczny: Bibliogr. 13 poz., rys., wykr., tab.

Twórcy

autor

Spórna-Kucab A.

• Cracow University of Technology, Department of Analytical Chemistry, Faculty of Chemical Engineering and Technology, Warszawska 24, 31-155 Cracow, Poland

autor

Wysoczańska J.

• Cracow University of Technology, Department of Analytical Chemistry, Faculty of Chemical Engineering and Technology, Warszawska 24, 31-155 Cracow, Poland

autor

Bartel A.

• Cracow University of Technology, Department of Analytical Chemistry, Faculty of Chemical Engineering and Technology, Warszawska 24, 31-155 Cracow, Poland

autor

Świergosz T.

• Cracow University of Technology, Department of Analytical Chemistry, Faculty of Chemical Engineering and Technology, Warszawska 24, 31-155 Cracow, Poland

autor

Szot D.

• Cracow University of Technology, Department of Analytical Chemistry, Faculty of Chemical Engineering and Technology, Warszawska 24, 31-155 Cracow, Poland

autor

Wybraniec S.

• Cracow University of Technology, Department of Analytical Chemistry, Faculty of Chemical Engineering and Technology, Warszawska 24, 31-155 Cracow, Poland

Bibliografia

• [1] Spórna-Kucab, A., Ignatova, S., Garrard, I., Wybraniec, S. “Versatile solvent systems for the separation of betalains from processed Beta vulgaris L. juice using counter-current chromatography”. Journal of chromatography B, 941 (2013): 54-61.
• [2] Spórna-Kucab, A., Garrard, I., Ignatova, S., Wybraniec, S. „New solvent systems for gradient counter-current chromatography in separation of betanin and its derivatives from processed Beta vulgaris L”. juice. Journal of Chromatography A, 1380, (2015):29-37.
• [3] Wybraniec, S. “A method for identification of diastereomers of 2-decarboxy-betacyanins and 2,17-bidecarboxy-betacyanins in reversed phase HPLC”. Analytical and Bioanalytical Chemistry, 389, (2007): 1611-1621.
• [4] Strack, D., Vogt, T., Schliemann, W. “Recent advances in betalain research”. Phytochemistry, 62, (2003): 247-269.
• [5] Wybraniec, S., Stalica, P., Jerz, G., Klose, B., Gebers, N., Winterhalter, P., Spórna, A., Szaleniec, M., Mizrahi Y. “Separation of polar betalain pigments from cacti fruits of Hylocereus polyrhizus by ion-pair high-speed countercurrent chromatography. Journal of Chromatography A, 1216, (2009): 6890-6899.
• [6] Jerz, G., Skotzki, T., Fiege, K., Winterhalter, P., Wybraniec, S. “Separation of betalains from berries of Phytolacca americana by ion-pair high-speed counter-current chromatography”. Journal of Chromatography A, 1190, (2008): 63-73.
• [7] Berthod, A. “Countercurrent chromatography”. Elsevier, Amsterdam, (2002): 1-37.
• [8] Ito, Y., Conway, W., D. “High-speed countercurrent chromatography”. Chemical Analysis, tom 132, (1993): 3-44.
• [9] Degenhardt, A., Winterhalter, P. “Isolation of natural pigments by high speed CCC”. Journal of Liquid Chromatography and Related Technologies, 24, (2001): 1745-1764.
• [10] Strack, D., Steglich, W., Wray, W. Methods in plant biochemistry, the University Press, Cambridge, 11, (1993): 422-447.
• [11] Jerz, G., Skotzki, T., Fiege, K., Winterhalter, P., Wybraniec, S. “Separation of betalains from berries of Phytolacca americana by ion-pair high-speed counter-current chromatography”. Journal of Chromatography A, 1190, (2008): 63-73.
• [12] Ito, Y. “Golden rules and pitfalls in selecting optimum conditions for high-speed counter-current chromatography”. Journal of Chromatography A, 1065, (2005): 145-168.
• [13] Wybraniec, S. “Effect of tetraalkylammonium salts on retention of betacyanins and decarboxylated betacyanins in ion-pair reversed- phase high-performance liquid chromatography”. Journal of Chromatography A, 1127, (2006), 70-75.

Uwagi

PL

Opracowanie ze środków MNiSW w ramach umowy 812/P-DUN/2016 na działalność upowszechniającą naukę.