New approach for n-hexadecane biodegradation by sol-gel entrapped bacterial cells

• Autorzy: Chernev G. , Christova N. , Kabaivanova L. , Nacheva L.

Identyfikatory

DOI

10.1515/eces-2018-0016

Warianty tytułu

PL

Nowe podejście do biodegradacji n-heksadekanu przez komórki bakteryjne uwięzione w materiale zol-żelowym

• Języki publikacji: EN

Abstrakty

EN

In this study sol-gel hybrid materials in the system SiO₂-chitosan (CS) - polyethylene glycol (PEG), as novel structures with potential application in bioremediation were investigated. The organic components - CS and PEG were used as structural modifiers for functionality improvement. The catabolic activity to n-hexadecane of Pseudomonas aeruginosa BN10 free and immobilized cells was estimated. The cell immobilization technique was employed to evaluate its efficiency on biodegradation and protective effect from high levels of hydrocarbons. The characteristics of obtained hybrid materials were investigated via X-ray Diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Scanning Electron Microscopy (SEM) and Atomic-force microscopy (AFM) analyses. The obtained results revealed that the organic part in the synthesized hybrids is important for microstructure and defined properties creation. The rate of n-hexadecane mineralization by the bacterial strain was influenced by variation in cell densities applied in the immobilization procedures. Semi-continuous processes with multiple xenobiotic supplies were carried out. The synthesized by the sol-gel method hybrid matrices proved to be suitable carriers for realizing an effective biodegradation process of n-hexadecane by Pseudomonas aeruginosa BN10. Biodegradation of 50 kg/m³ of n-hexadecane was realized by free cells. Significantly greater quantity (150 kg/m³) was mineralized for 15 active cycles by entrapped bacterial cells. Biodegradation process with gradual increase of xenobiotic concentration reaching 30 kg/m³ for 120 h was also accomplished.

Słowa kluczowe

EN

biodegradation; n-hexadecane; hybrids; sol-gel; Pseudomonas aeruginosa

PL

biodegradacja; n-heksadekan; hybrydy; zol-żel; Pseudomonas aeruginosa

• Wydawca: Towarzystwo Chemii i Inżynierii Ekologicznej
• Czasopismo: Ecological Chemistry and Engineering. S
• Rocznik: 2018
• Tom: Vol. 25, nr 2
• Strony: 243--253
• Opis fizyczny: Bibliogr. 29 poz., wykr., rys.

Twórcy

autor

Chernev G.

• Department of Silicate Technology, University of Chemical Technology and Metallurgy, 8 Kliment Ohridski blvd., Sofia 1756, Bulgaria

autor

Christova N.

• Institute of Microbiology “Stephan Angeloff”, Bulgarian Academy of Sciences, “Acad. G. Bonchev” str., bl. 26, Sofia 1113, Bulgaria

autor

Kabaivanova L.

• Institute of Microbiology “Stephan Angeloff”, Bulgarian Academy of Sciences, “Acad. G. Bonchev” str., bl. 26, Sofia 1113, Bulgaria

autor

Nacheva L.

• Institute of Microbiology “Stephan Angeloff”, Bulgarian Academy of Sciences, “Acad. G. Bonchev” str., bl. 26, Sofia 1113, Bulgaria

Bibliografia

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Uwagi

PL

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2018).