Application of solid-phase extraction (SPE) and high-performance liquid chromatography (HPLC) and comparison of both detection techniques (DAD and FLD) to analyse nesfatin-1 in fetal bovine serum samples

• Autorzy: Tuzimski Tomasz , Szubartowski Michał

Identyfikatory

DOI

10.1556/1326.2022.01076

• Języki publikacji: EN

Abstrakty

EN

In this study, we propose a simple, cost-effective, and sensitive high-performance liquid chromatography with both detection techniques such as diode-array detection and fluorescence detection (HPLC-DAD-FLD) for the determination of nesfatin-1 in fetal bovine serum samples. The limit of detection (LOD) and limit of quantification (LOQ) for nesfatin-1 were set at satisfactory values in the range 0.22–0.35 mg mL⁻¹ and in the range 0.67–1.05 mg mL⁻¹, respectively (at two different wavelengths (DAD) and at four different wavelengths (FLD)). Analyte concentrations were determined as the average value from fetal bovine serum matrix samples. The preliminary results show that the SPE procedure on Isolute Si-TsOH (SCX-3) could be used for further nesfatin-1 analyses in human serum samples. Both the SPE technique, chromatographic analysis with gradient elution mode and detection technique are fast and convenient.

Słowa kluczowe

EN

nesfatin-1; fetal bovine serum samples; high-performance liquid chromatography; HPLC; fluorescence detector; FLD; solid-phase extraction; SPE; Isolute Si-TsOH; SCX-3; PL Rapide Aqua L column

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2023
• Tom: Vol. 35, no. 3
• Strony: 286--292
• Opis fizyczny: Bibliogr. 18 poz., rys., tab., wykr.

Twórcy

autor

Tuzimski Tomasz

• Department of Physical Chemistry, Medical University of Lublin, Chod zki 4a, 20-093, Lublin, Poland

• https://orcid.org/0000-0003-3729-3196

autor

Szubartowski Michał

• Student Research Group at the Department of Physical Chemistry, Chair of Chemistry, Faculty of Pharmacy, Medical University of Lublin, Chod zki 4a, 20-093, Lublin, Poland

Bibliografia

• 1. Oh-I, S.; Shimizu, H.; Satoh, T.; Okada, S.; Adachi, S.; Inoue, K.; Eguchi, H.; Yamamoto, M.; Imaki, T.; Hashimoto, K.; Tsuchiya, T.; Monden, T.; Horiguchi, K.; Yamada, M.; Mori, M. Nature 2006, 443, 709–12.
• 2. Leung, A.K.-W.; Ramesh, N.; Vogel, C.; Unniappan, S. Adv. Protein Chem. Struct. Biol. 2019, 116, 91–133. https://doi.org/10.1016/bs.apcsb.2019.02.001.
• 3. Feijóo-Bandín, S.; Rodríguez-Penas, D.; García-Rúa, V.; Mosquera-Leal, A.; González-Juanatey, J. R.; Lago, F. Endocrine 2016, 52, 11–29. https://doi.org/10.1007/s12020-015-0819-z.
• 4. Dore, R.; Levata, L.; Lehnert, H.; Schulz, C. J. Endocrinol. 2017, 232(1), R45–65; https://doi.org/10.1530/JOE-16-0361.
• 5. Miura, K.; Titani, K.; Kurosawa, Y.; Kanai, Y. Biochem. Biophys. Res. Comm. 1992, 187, 375–80.
• 6. Kmiecik, A. M.; Dzięgiel, P.; Podhorska-Okołów, M. Int. J. Mol. Sci. 2021, 22, 8313. https://doi.org/10.3390/ijms22158313.
• 7. Shimizu, H.; Oh-I, S.; Hashimoto, K.; Nakata, M.; Yamamoto, S.; Yoshida, N.; Eguchi, H.; Kato, I.; Inoue, K.; Satoh, T.; Okada, S.; Yamada, M.; Yada, T.; Mori, M. Endocrinol 2009, 150, 662–71.
• 8. Shimizu, H.; Oh-I, S.; Okada, S.; Mori, M. Endocr. J. 2009, 56(4), 537–54.
• 9. Fernandez-Fernandez, R.; Martini, A. C.; Navarro, V. M.; Castellano, J. M.; Dieguez, C.; Aguilar, E.; Pinilla, L.; Tena-Sempere, M. Mol. Cell. Endocrinol. 2006, 254-255, 127–32.
• 10. Garcia-Galiano, D.; Navarro, V. M.; Gaytan, F.; Tena-Sempere, M. J. Mol. Endocrinol. 2010, 45, 281–90.
• 11. Zhang, H.; Qi, C.; Wang, A.; Yao, B.; Li, L.; Wang, Y.; Xu, Y. J. Exp. Clin. Cancer Res. 2013, 32, 1–8.
• 12. Yamada, M.; Horiguchi, K.; Umezawa, R.; Hashimoto, K.; Satoh, T.; Ozawa, A.; Shibusawa, N.; Monden, T.; Okada, S.; Shimizu, H.; Mori, M. Endocrinology 2010, 151, 2494–503.
• 13. Ayada, C.; Toru, Ü.; Korkut, Y. Hippokratia 2015, 19, 4–10.
• 14. Zhang, J.; Späth, S. S.; Marjani, S. L.; Zhang, W.; Pan, X. Precision Clin. Med. 2018, 1(1), 29–48. https://doi.org/10.1093/pcmedi/pby007.
• 15. Zhao, J.; Yun, X.; Ruan, X.; Chi, J.; Yu, Y.; Li, Y.; Zheng, X.; Gao, M. Onco. Targets Ther. 2019, 12, 1309–18.
• 16. Cho, J. M.; Moon, K. T.; Lee, H. J.; Shin, S. C.; Choi, J. D.; Kang, J. Y.; Yoo, T. K. Medicine 2020, 99, e19597.
• 17. Skorupska, A.; Lenda, R.; Ożyhar, A.; Bystranowska, D. Int. J. Mol. Sci. 2021, 22, 5687. https://doi.org/10.3390/ijms22115687.
• 18. ICH, Guidelines. Technical Requirements for the Registration of Pharmaceutical for Human Use, Validation of Analytical Procedures: Text and methodologyQ2 (R1); International Conference on Harmonization: Geneva, Switzerland, 2005.