High-throughput monospin extraction for quantification of cardiovascular drugs in serum coupled to high-performance liquid chromatography–mass spectrometry

• Autorzy: Namera Akira , Saito Takeshi , Seki Yoshimoto , Mizutani Taro , Murata Kazuhiro , Nagao Masataka

Identyfikatory

DOI

10.1556/1326.2018.00493

• Języki publikacji: EN

Abstrakty

EN

A novel method coupling spin column extraction with high-performance liquid chromatography–mass spectrometry was developed for simultaneous extraction of β-blockers and calcium channel blockers from human serum. Sample loading, washing, and elution were accomplished via centrifugation of the column, in which mixed-mode monolithic silica bonded to a C18 reversed phase, and a cation-exchange phase was packed in a spin column. The serum sample (0.2 mL) pH was adjusted to 3 and the analytes adsorbed onto the column were eluted with 0.1 mL MeOH containing 2% NH3. The recoveries of the tested drugs were 76–108%. A linear curve was observed up to a concentration of 500 ng/mL of the target drugs in serum (r2 > 0.996). The intra-day relative standard deviations at three different concentrations were 0.6–9.6%. The limits of detection were 2 ng/mL. The proposed method was successfully applied to clinical and forensic cases.

Słowa kluczowe

EN

β-blockers; calcium channel blockers; monolithic silica; solid-phase extraction; mix-mode

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2019
• Tom: Vol. 31, no. 4
• Strony: 294--298
• Opis fizyczny: Bibliogr. 21 poz., rys., tab.

Twórcy

autor

Namera Akira

• Department of Forensic Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, Japan

autor

Saito Takeshi

• Department of Emergency and Critical Care Medicine, Tokai University School of Medicine, Kanagawa 259-1143, Japan

autor

Seki Yoshimoto

• Ibaraki Prefectural Central Hospital, Ibaraki 309-1703, Japan

autor

Mizutani Taro

• Chikusei City Medical Director, Ibaraki 308-8616, Japan

autor

Murata Kazuhiro

• Department of Forensic Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, Japan

autor

Nagao Masataka

• Department of Forensic Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, Japan

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