Simultaneous HPLC-DAD analysis of five flavonoids in diabetic rat plasma and its application in the study of pharmacokinetics

• Autorzy: Tang D , Yin Y , Zhang Z. , Gao Y. , Wei Y , Chen Y , Han Y.
• Języki publikacji: EN

Abstrakty

EN

A HPLC-DAD method for simultaneous analysis of five flavonoids (rutin, quercitrin, quercetin, kaempferol, and isorhamnetin) in diabetic rat plasma has been developed and validated. Separation of the five flavonoids was accomplished on a C 18 column (250 mm × 4.6 mm i.d., 5-µm particle) and detection was performed at 350 nm. The best resolution was achieved with a methanol-0.1% formic acid gradient at a flow rate of 1.0 mL min −1 . The correlation coefficients for all the calibration plots ( r > 0.999) showed linearity was good over the range tested. The relative standard deviation of the method was less than 7% and 10% for intra- and inter-day assays, and average recovery was between 77.2 and 99.2%. Sensitivity was high and detection limits were between 0.006 and 0.02 µg mL −1 . The method has been successfully used to determine drug concentrations in diabetic rat plasma samples and the pharmacokinetics of the drugs.

Słowa kluczowe

EN

high-performance liquid chromatography; Ginkgo biloba extract; pharmacokinetics; rutin; quercitrin; quercetin; kaempferol; isorhamnetin

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2009
• Tom: Vol. 21, no. 3
• Strony: 483--497
• Opis fizyczny: Bibliogr. 29 poz., rys., tab.

Twórcy

autor

Tang D

• Ministry of Education Key Laboratory of Drug Quality Control and Pharmacovigilance Nanjing Jiangsu 210009 China
• China Pharmaceutical University Center for Instrumental Analysis Nanjing Jiangsu 210009 China

autor

Yin Y

• Xuzhou Medical College Department of Pharmaceutical Analysis Xuzhou Jiangsu 221004 China

autor

Zhang Z.

• Ministry of Education Key Laboratory of Drug Quality Control and Pharmacovigilance Nanjing Jiangsu 210009 China
• China Pharmaceutical University Center for Instrumental Analysis Nanjing Jiangsu 210009 China

autor

Gao Y.

• Xuzhou Medical College Department of Pharmaceutical Analysis Xuzhou Jiangsu 221004 China

autor

Wei Y

• Xuzhou Medical College Department of Pharmaceutical Analysis Xuzhou Jiangsu 221004 China

autor

Chen Y

• Xuzhou Medical College Department of Pharmaceutical Analysis Xuzhou Jiangsu 221004 China

autor

Han Y.

• Xuzhou Medical College Department of Pharmaceutical Analysis Xuzhou Jiangsu 221004 China

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