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Cytotoxic Test of Octopus cyanea Ink Extract

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Identyfikatory
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Marine biodiversity of Papua is very high, but unfortunately research and utilization in medicine are limited. Many species of flora and fauna are used in ethopharmacology as drugs but without scientific explanation, e.g. octopus (Octopus cyanea), found in the Northern Papua Sea. The aim of this research was to test the toxicity of the ethanol octopus ink. The toxicity was tested with the Brine Shrimp Lethality Test (BSLT) method. The samples were collected from the sea of Biak island in West Papua, extracted with ethanol, ethyl acetate, and dichloromethane then tested the toxicity for Artemia salina leach larvae. The toxicity test involved concentrations of 1000, 750, 500, 250, 100, 75, 50, and 25 ppm. The result showed that ethanol extract of octopus ink has LC50 of 290 ppm, ethyl acetate extract has LC50 of 947 ppm, whereas dicholoromethane extract – 2190 ppm.
Słowa kluczowe
Rocznik
Strony
144--152
Opis fizyczny
Bibliogr. 22 poz., rys., tab.
Twórcy
  • Departement of Marine Scinece, Cenderawasih University, Jayapura Papua, Indonesia
  • Departement of Pharmacy, Cenderawasih University, Jayapura Papua, Indonesia
  • Departement of Chemistry, Cenderawasih University, Jayapura Papua, Indonesia
  • Departement of Pharmacy, Cenderawasih University, Jayapura Papua, Indonesia
  • Departement of Pharmacy, Cenderawasih University, Jayapura Papua, Indonesia
  • Departement of Chemistry, Cenderawasih University, Jayapura Papua, Indonesia
Bibliografia
  • 1. Doyle A., Griffiths J. B. 2000. Cell and tissue culture for medical research. John Willey and Sons, ltd, New York.
  • 2. Fadhli H., Teruna H.Y., Jose C. 2012. Toxicity test of Puai Basung (Alstonia spatula BL) bark extract using the brine shrimp lethality test method. J Ind Che Acta, 3(1),10–15. (in Indonesia).
  • 3. Fitahia E. M., Raheriniaina C. E., Bazin M. A., Huvelin J. M., Loge C., Ranaivoson E., Nazih H. 2015. Anti-proliferative and pro-apoptotic effect of dichloromethane extract of Octopus vulgaris by-product on human breast cancer cell lines. Weste Biomass Major, 6, 237–242.
  • 4. Geran R. I., Greenberg N. H., Macdonald M. M., Schumacher A. M., Abbott B. J. 1992. Protocol for screening chemical agents and natural product against animal tumurs and other biological system. Cancer Chemother, 3, 59–61.
  • 5. Juniarti, Osmeli D., Yuhernita. 2009. Chemical compounds, toxicity test (Brine shrimp lethality test) and antioxidants (1,1-diphenyl-2-pikrilhydrazyl) from saga leaf extract (Abrus pecatorius L). Makara sains, 13,50–54. (in Indonesia).
  • 6. Karthigayan S., Balasubashini M. S., Sengottuvelan M., Balasubramanian T., Somasundaram S.T. 2006. Anticancer prinsiples from salivary extract of Octopus ageina. International Journal of Cancer Research, 2.
  • 7. Kuba M., Meisel D. V., Byrne R. A., Griebel U., Mather J. A. 2003. Looking at play in Octopus vulgaris. Berliner Palaobiol Abh, 3, 163–169.
  • 8. Lisa E. M., Maio A. D., Moroz L. I., Moccia F., Menella M. R. F., Cosmo, A. I. 2012. Characterization of novel cytoplasmic PARP in the brain of Octopus vulgaris. Bio Bull, 222, 171–181.
  • 9. Martinez S. C. 2013. Coccidiosis and molecular basis of the immune response of common octopus. Thesis. Vigo Spanyol: Universida de Vigo, De Ecologia Biologia Animal.
  • 10. Moeljopawiro S., Nuringtyas T. R., Noveriza R., Trisilawati O. 2007. Identification of the bioactive fraction of anti breast cancer and uterine cancer and contaminant microbes in three red fruit vetasetas (Pandanus Conoideus Lam). Kerjasama KemitraanPenelitian Pertanian dengan Perguruan Tinggi. Jakarta, Universitas Gadjah Mada. (in Indonesia).
  • 11. Mardiyaninsih A., Ismiyati N. 2014. Cytotoxic activity of ethanol extract of avocado leaves (Persea Americana. Mill) on cervical cancer cells hela. Trad Med, 19(1), 24–28. (in Indonesia).
  • 12. Mardanny M.T. 2015. Phytochemical screening and testing of cytotoxic activity from ant nest plants (Myrmecodia Beccarii Hook.F.) from Merauke Regency. Universitas Cenderawasih, Jayapura (in Indonesia).
  • 13. Martinez S. C. 2013. Coccidiosis and molecular basis of the immune response of common octopus. Thesis. Vigo Spanyol: Universida de Vigo, De Ecologia Biologia Animal.
  • 14. McLaughlin J.L. 1991. Crown gall tumours on potato disc and brine shrimp lethality: two simple bioassay for higher plant screening and fractionation. Methods in plant biochemistry, 6(1), 1–30
  • 15. Meyer B.N., Ferigni N.R., Putnam J.E., Jaconsen L.B., Nichols D.F., Mc laughin J. L. 1982. Brine shimp a convenient general bioassay for active plant constituents. Planta Medica, 45, 31–34.
  • 16. Murphy M. C. 1999. Plant products as antimicrobial agents. Clin Microbiol Rev, 12,564–582l
  • 17. Nurhayati A. P. D., Abdulgani N., Febrianto R. 2006. Toxicity test of Eucheuma alvarezii extract against Artemia salina as a preliminary study of anti-cancer potential. Akta Kimindo, 41–46. (in Indonesia).
  • 18. Paruntu C. P., Boneka F. B., Talare S. L. 2009. Octopus (Cephalopoda) from Sangihe Waters, North Sulawesi. Ekoton, 9(2), 13–14. (in Indonesia).
  • 19. Posangi J., Juliatri, Bara R., Tairas J., Wuisan J. 2013. Test the antibacterial effects of squid ink (Loligo sp.) On tooth root canal bacteria. Skripsi. Manado: Fakultas Kedokteran. (in Indonesia).
  • 20. Purwantini I., Setyowati E. P., Hertiani T. 2002. Toxicity test of ethanol extract of fruit seeds, daun mahkota dewa (Phaleria marcocarpa) against Artemia Salina Leach and active thin layer chromatography extract. Majalah Farmasi Indonesia, 101–106. (in Indonesia).
  • 21. Ren W., Qiao Z., Wang H., Zhu L., Zhang L., 2003. Flavonoids: promising anticancer agents. Medicinal Research Review, 23 (4), 519–53.
  • 22. Robinson T. 1995. High levels of organic plant content. ITB, Bandung. (in Indonesia).
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-6af53f95-b223-4153-95d6-1096df529758
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