Gas chromatographic determination of phenol in fish tissues as a phenyl acetate derivative following solvent extraction of acidified samples

• Autorzy: Kim H. , Kim Y. , Park S. , Lee J. , Choi J.

Identyfikatory

DOI

10.1556/1326.2018.00529

• Języki publikacji: EN

Abstrakty

EN

This study aimed to develop a chromatographic method to quantitatively determine phenol in fish tissues. This method involves solvent extraction of acidified samples, followed by derivatization to phenyl acetate and analysis with gas chromatography coupled with mass spectrometry (GC–MS). Phenol in a representative tissue sample (belly, gill, or renal tubules), which was homogenized with 2 N sulfuric acid, was extracted with ethyl acetate and derivatized to phenyl acetate using acetic anhydride and K2CO3 in water. An n-butyl acetate extract was injected into the GC–MS. The linearity (r2) of the calibration curve was greater than 0.996. The analytical repeatability, which is expressed as the relative standard deviation, was less than 6.14%, and the recovery was greater than 96.3%. The method detection limit and the limit of quantitation were 8.0 μg/kg and 26 μg/kg, respectively. The proposed method is also applicable to the analysis of other biological tissues for phenol and its analogs, such as pentachlorophenol.

Słowa kluczowe

EN

acetylation; fish; GC-MS; phenol; solvent extraction

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2020
• Tom: Vol. 32, no. 1
• Strony: 64--67
• Opis fizyczny: Bibliogr. 14 poz., rys.

Twórcy

autor

Kim H.

• Kangwon National University, Chuncheon, Kangwon-do 24341, Republic of Korea

autor

Kim Y.

• Kangwon National University, Chuncheon, Kangwon-do 24341, Republic of Korea

autor

Park S.

• Kangwon National University, Chuncheon, Kangwon-do 24341, Republic of Korea

autor

Lee J.

• Natural and Human Co., Ltd., Bugwon-ro, Wonju, Kangwon-do 26424, Republic of Korea

autor

Choi J.

• Kangwon National University, Chuncheon, Kangwon-do 24341, Republic of Korea

Bibliografia

• [1.] Bruce, R. M.; Santodonato, J.; Neal, M. W. Toxicol. Ind. Health 1987, 3, 535.
• [2.] US EPA, Toxicological Review of Phenol (CAS No. 108-95-2) Integrated Risk Information System (IRIS), National Center for Environmental Assessment, Office of Research and Development, Washington, DC, USA, 2002.
• [3.] ATSDR, Toxicological Profile for Phenol Public Health Service, U.S. Department of Health Services, Atlanta, GA, USA, 2008.
• [4.] Razani, H.; Nanba, K.; Murachi, S. B. Jpn. Soc. Sci. Fish. 1986, 52, 1547.
• [5.] Saha, N. C.; Bhunia, F.; Kaviraj, A. B. Environ. Contam. Tox. 1999, 63, 195.
• [6.] Ballesteros, E.; Gallego, M.; Valcarcel, M. J. Chromatogr. 1990, 518, 59.
• [7.] Llompart, M.; Lourido, M.; Landín, P.; García-Jares, C.; Cela, R. J. Chromatogr. A 2002, 963, 137.
• [8.] Bagheri, H.; Saber, A.; Mousavi, S. R. J. Chromatogr. A 2004, 1046, 27.
• [9.] Faraji, H. J. Chromatogr. A 2005, 1087, 283.
• [10.] Park, S.; Kim, Y.; Jung, S.; Kim, H. Kor. J. Environ. Agric. 2017, 36, 63.
• [11.] Stahl, L. L.; Snyder, B. D.; Olsen, A. R.; Pitt, J. L. Environ. Monit. Assess. 2009, 150, 3.
• [12.] US EPA Method 1625C Semivolatile Organic Compounds by Isotope Dilution GCMS Office of Science and Technology Engineering and Analysis Division, Washington, DC, USA, 1989.
• [13.] Kang, Y. W.; Ahn, J. E.; Suh, J. H.; Park, S. H.; Yoon, H. J. J. Food Hyg. Safety 2014, 29, 312.
• [14.] US EPA Definition and Procedure for the Determination of the Method Detection Limit, Revision 2 Office of Water, Washington, DC, USA, 2016.

Uwagi

PL

Opracowanie rekordu ze środków MNiSW, umowa Nr 461252 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2020).