TLC—Densitometric Determination of Sulfasalazine and Its Possible Impurities in Pharmaceutical Preparations

• Autorzy: Kwiecień A. , Piątek K. , Żmudzki P. , Krzek J.
• Języki publikacji: EN

Abstrakty

EN

A simple, selective, and sensitive thin-layer chromatographic—densitometric method has been developed for the determination of sulfasalazine besides its possible impurities in pharmaceutical preparations. The mobile phase was composed of ethyl acetate—methanol—ammonia 25% 10:7:3 (υ/υ/υ), and the stationary phase was aluminum plates precoated with silica gel 60 F254 that enabled to obtain well resolved peaks of sulfasalazine and its impurities. The developed chromatograms were analyzed densitometrically at λ = 360 nm. RF values and ultraviolet (UV) spectra were used to identify the compounds. The developed method is highly sensitive (limit of detection [LOD] = 17.11 ng spot−1, limit of quantitation [LOQ] = 51.84 ng spot−1), precise (relative standard deviation [RSD] = 1.43%–4.28%), and accurate (RSD = 1.64%–4.27%). The linearity of the method was checked within the range 20–120 ng spot−1. The method was successfully applied for the determination of sulfasalazine in pharmaceutical preparations besides its impurities. The structures of impurities present in the standard substance and in pharmaceutical preparations were established by ultra-performance liquid chromatography—tandem mass spectrometry (UPLC—MS/MS) technique.

Słowa kluczowe

EN

thin-layer chromatography; drug analysis; sulfasalazine; impurities

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2015
• Tom: Vol. 27, no. 4
• Strony: 623--635
• Opis fizyczny: Bibliogr. 11 poz., rys., tab.

Twórcy

autor

Kwiecień A.

• Department of Inorganic and Analytical Chemistry, Jagiellonian University, Medical College, Faculty of Pharmacy, Medyczna 9, 30-688, Kraków, Poland

autor

Piątek K.

• Department of Inorganic and Analytical Chemistry, Jagiellonian University, Medical College, Faculty of Pharmacy, Medyczna 9, 30-688, Kraków, Poland

autor

Żmudzki P.

• Department of Inorganic and Analytical Chemistry, Jagiellonian University, Medical College, Faculty of Pharmacy, Medyczna 9, 30-688, Kraków, Poland

autor

Krzek J.

• Department of Inorganic and Analytical Chemistry, Jagiellonian University, Medical College, Faculty of Pharmacy, Medyczna 9, 30-688, Kraków, Poland

Bibliografia

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