LC-MS/MS method for the quantification of withaferin-A in plant extracts of Withania spp.

• Autorzy: Mirjalili M. H. , Navarro A. , Hernández L. , Jáuregui O. , Bonfill M.
• Języki publikacji: EN

Abstrakty

EN

The quantification of the main withanolide, withaferin-A, in plant extracts of Withania somnifera and Withania coagulans using high-performance liquid chromatography (HPLC)-mass spectrometry (ESI-MS/MS) is described. HPLC was used in combination with a triple quadrupole mass spectrometer in multiple reaction monitoring (MRM) mode. Additionally, mass exact determination of withaferin-A was achieved using a hybrid high-resolution mass spectrometer (Q-TOF). A combination of acetonitrile and water as the mobile phase using gradient elution was found to be successful in separating the compound of interest from the other main components. The limit of detection (LOD) in the plants was 6 ng/g and the total analysis time was 10 min.

Słowa kluczowe

EN

Withania somnifera; Withania coagulans; withaferin-A; liquid chromatography; mass spectrometry; exact mass measurement

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2013
• Tom: Vol. 25, no. 4
• Strony: 745--754
• Opis fizyczny: Bibliogr. 14 poz., rys., tab.

Twórcy

autor

Mirjalili M. H.

• Shahid Beheshti University Medicinal Plants and Drugs Research Institute G.C., Evin Tehran Iran

autor

Navarro A.

• National Autonomous University of Mexico Department of Food and Biotechnology, Faculty of Chemistry Ciudad de México 04510 Mexico

autor

Hernández L.

• Universidad Autónoma Metropolitana-Xochimilco Departamento de Sistemas Biológicos Calz. del Hueso 1100, Col. Villa Quietud 04960 México, D. F. México

autor

Jáuregui O.

• Universitat de Barcelona Scientific & Technological Centers (CCiTUB) c/Baldiri i Reixach 10-12 08028 Barcelona Spain

autor

Bonfill M.

• Universitat de Barcelona Laboratori de Fisiologia Vegetal, Facultat de Farmàcia Avda. Joan XXIII s/n 08028 Barcelona Spain

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