PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
Tytuł artykułu

LC-MS/MS method for the quantification of withaferin-A in plant extracts of Withania spp.

Identyfikatory
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The quantification of the main withanolide, withaferin-A, in plant extracts of Withania somnifera and Withania coagulans using high-performance liquid chromatography (HPLC)-mass spectrometry (ESI-MS/MS) is described. HPLC was used in combination with a triple quadrupole mass spectrometer in multiple reaction monitoring (MRM) mode. Additionally, mass exact determination of withaferin-A was achieved using a hybrid high-resolution mass spectrometer (Q-TOF). A combination of acetonitrile and water as the mobile phase using gradient elution was found to be successful in separating the compound of interest from the other main components. The limit of detection (LOD) in the plants was 6 ng/g and the total analysis time was 10 min.
Rocznik
Strony
745--754
Opis fizyczny
Bibliogr. 14 poz., rys., tab.
Twórcy
  • Shahid Beheshti University Medicinal Plants and Drugs Research Institute G.C., Evin Tehran Iran
autor
  • National Autonomous University of Mexico Department of Food and Biotechnology, Faculty of Chemistry Ciudad de México 04510 Mexico
  • Universidad Autónoma Metropolitana-Xochimilco Departamento de Sistemas Biológicos Calz. del Hueso 1100, Col. Villa Quietud 04960 México, D. F. México
autor
  • Universitat de Barcelona Scientific & Technological Centers (CCiTUB) c/Baldiri i Reixach 10-12 08028 Barcelona Spain
autor
  • Universitat de Barcelona Laboratori de Fisiologia Vegetal, Facultat de Farmàcia Avda. Joan XXIII s/n 08028 Barcelona Spain
Bibliografia
  • [1] G.L. Gupta and A.C. Rana, Pharmacogn. Rev., 1, 129 (2007)
  • [2] P. Bargagna-Mohan, A. Hamza, Y-E. Kim, Y.K. Ho, N. Mor-Vaknin, N. Wendschlag, J. Liu, R.M. Evans, D.M. Markovitz, C-G. Zhan, K.B. Kim, and R. Mohan, Chemistry & Biology, 14, 623 (2007)
  • [3] R.S. Sangwan, N.D. Chaurasiya, L.N. Misra, P. Lal, G.C. Uniyal, R. Sharma, N.S. Sangwan, K.A. Suri, G.N. Qazi, and R. Tuli, Current Sci., 86, 461 (2004)
  • [4] M. Ganzera, M.I. Choudhary, and I.A. Khan, Fitoterapia, 74, 68 (2003)
  • [5] N.D. Chaurasiya, G.C. Uniyal, P. Lal, L. Misra, N.S. Sangwan, R. Tuli, and R.S. Sangwan, Phytochem. Anal., 19, 148 (2008)
  • [6] A.B. Ray and M. Gupta, Prog. Chem. Org. Nat. Prod., 63, 1 (1994)
  • [7] L. Dinan, J. Harmatha, and R. Lafont, J. Chromatogr. A, 935, 105 (2001)
  • [8] M.H. Mirjalili, S.M.F. Tabatabaei, H. Alizadeh, A. Ghassempour, and F. Mirzajani, Nat. Prod. Commun., 4, 337 (2009)
  • [9] R.K. Khajuria, K.A. Suri, R.K. Gupta, N.K. Satti, M. Amina, O.P. Suri, and G.N. Qazi, J. Sep. Sci., 27, 541–546 (2004)
  • [10] B. Kaufmann, S. Souverain, S. Cherkaoui, P. Christen, and J.L. Veuthey, Chromatographia, 56, 137 (2002)
  • [11] M.H. Mirjalili, S.M. Fakhr-Tabatabaei, M. Bonfill, H. Alizadeh, R.M. Cusido, A. Ghassempour, and J. Palazon, Engineering in Life Sci., 9, 197 (2009)
  • [12] V. Sharma, A.P. Gupta, P. Bhandari, R.C. Gupta, and B. Singh, Chromatographia, 66, 801 (2007)
  • [13] M. Bandyopadhyay, S. Jha, and D. Tapfer, Plant Cell Rep., 26, 599 (2007)
  • [14] M. Sharada, A. Ahuja, K.A. Suri, S.P. Vij, R.K. Khajuria, V. Vermaand, and A. Kumar, Biol. Plantarum, 51, 161 (2007)
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-66dff2d0-dba2-43b8-8cdb-f2f944d468dd
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.