Separation of ampicillin on polar-endcapped phase : Development of the HPLC method to achieve its correct dosage in cardiac surgery

• Autorzy: Kubickova Vendula , Racova Zuzana , Strojil Jan , Santavy Petr , Urbanek Karel

Identyfikatory

DOI

10.1556/1326.2021.00957

• Języki publikacji: EN

Abstrakty

EN

The accurate, simple, and selective reversed phase high performance liquid chromatography (RP-HPLC) has been established and validated for the determination of an antibiotic ampicillin (AMP) in human blood plasma. The SPE extraction was used for the sample preparation. Chromatographic separation was accomplished by a mobile phase containing 15 mM monopotassium phosphate solution of pH 3.3 and methanol (75:25, v/v) in an isocratic mode at a flow rate of 1.4 mL min⁻¹ at 30 °C. The separation was evaluated on a column with a new polar-endcapped C₁₈ stationary phase Arion® Polar C18 or well-known phase Luna® Omega Polar C18. Excellent linearity (R² 0.9998) was shown over range 10–300 mg L⁻¹ with mean percentage recovery 90%. Peak shapes were symmetrical in both columns, Arion® Polar C18 and Luna® Omega Polar C18, with asymmetry factor of 1.0 and 1.4, tailing factor of 1.0 and 1.2, and retention factor of 4.6 and 5.6, respectively. The Arion® Polar C18 was almost 1.4-fold more effective than Luna® Omega Polar C18 phase. The LOQ for ampicillin was achieved 10 mg L⁻¹ for Luna® Omega Polar C18 and 5 mg L⁻¹ for Arion® Polar C18 using 20 µL of a solution containing 0.24 mg mL⁻¹ of cephalexin as an internal standard. A number of articles dealing with the determination of ampicillin is limited, therefore, this study showed the HPLC method suitable for the determination of AMP in human blood plasma from patient who underwent elective cardiac surgical revascularization. In addition, the determination of AMP was also performed for the first time using an Arion® Polar C18 column, which effectively separated AMP from other compounds present in human blood plasma. This new polar-endcapped phase can help in separation of polar antibiotics or other polar compounds, which are unsuccessfully separated on conventional C₁₈ column, and thus can help during method development.

Słowa kluczowe

EN

ampicillin; polar column; separation

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2023
• Tom: Vol. 35, no. 1
• Strony: 1--9
• Opis fizyczny: Bibliogr. 43 poz., tab., rys., wykr.

Twórcy

autor

Kubickova Vendula

• Department of Pharmacology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Olomouc, Czech Republic

autor

Racova Zuzana

• Department of Pharmacology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Olomouc, Czech Republic

• https://orcid.org/0000-0001-9478-9513

autor

Strojil Jan

• Department of Pharmacology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Olomouc, Czech Republic

autor

Santavy Petr

• Department of Cardiac Surgery, University Hospital Olomouc, Olomouc, Czech Republic

autor

Urbanek Karel

• Department of Pharmacology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Olomouc, Czech Republic

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Uwagi

Opracowanie rekordu ze środków MEiN, umowa nr SONP/SP/546092/2022 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2022-2023).