Imaging of hypoxia in small animals with 18F fluoromisonidasole

• Autorzy: Kilian K. , Rogulski Z. , Cheda Ł. , Drzał A. , Gerszewska M. , Cudny M. , Elas M.

Identyfikatory

DOI

10.1515/nuka-2016-0037

• Konferencja: PLASMA-2015 International Conference on Research and Applications of Plasmas (7-11 September 2015 ; Warsaw, Poland)
• Języki publikacji: EN

Abstrakty

EN

A method of automated synthesis of [18F]fluoromisonidazole ([18F]FMISO) for application in preclinical studies on small animals was presented. A remote-controlled synthesizer Synthra RNplus was used for nucleophilic substitution of NITTP (1-(2'-nitro-1'-imidazolyl)-2-O-tetrahydropyranyl-3-O-toluenesulfonyl-propanediol) with 18F anion. Labeling of 5 mg of precursor was performed in anhydrous acetonitrile at 100°C for 10 min, and the hydrolysis with HCl was performed at 100°C for 5 min. Final purifi cation was done with high-performance liquid chromatography (HPLC) and the radiochemical purity of radiotracer was higher than 99%. Proposed [18F]FMISO synthesis was used as a reliable tool in studies on hypoxia in Lewis lung carcinoma (LLC) in mouse models.

Słowa kluczowe

EN

18F-fluoromisonidazole; radiopharmaceuticals; hypoxia imaging

• Wydawca: Institute of Nuclear Chemistry and Technology
• Czasopismo: Nukleonika
• Rocznik: 2016
• Tom: Vol. 61, No. 2
• Strony: 119--223
• Opis fizyczny: Bibliogr. 18 poz., rys.

Twórcy

autor

Kilian K.

• Heavy Ion Laboratory, University of Warsaw, 5A Pasteura Str., 02-093 Warsaw, Poland, Tel.: [phone]

autor

Rogulski Z.

• Faculty of Chemistry, University of Warsaw, 1 Pasteura Str., 02-093 Warsaw, Poland

autor

Cheda Ł.

• Faculty of Chemistry, University of Warsaw, 1 Pasteura Str., 02-093 Warsaw, Poland

autor

Drzał A.

• Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 7 Gronostajowa Str., 30-387 Krakow, Poland

autor

Gerszewska M.

• Faculty of Physics, University of Warsaw, 5 Pasteura Str., 02-093 Warsaw, Poland

autor

Cudny M.

• Faculty of Physics, University of Warsaw, 5 Pasteura Str., 02-093 Warsaw, Poland

autor

Elas M.

• Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 7 Gronostajowa Str., 30-387 Krakow, Poland

Bibliografia

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• 3. Ackerman, D., & Simon, M. C. (2014). Hypoxia, lipids, and cancer: surviving the harsh tumor microenvironment. Trends Cell. Biol., 24(8), 472–477.
• 4. Brown, J. M. (2007). Tumor hypoxia in cancer therapy. H. Sies, & B. Brune (Eds.) Methods in enzymolology.Vol. 435 (pp. 297–321). Academic Press.
• 5. Nagelkerke, A., Bussink, J., Mujcic, H., Wouters, B. G., Lehmann, S., Sweep, F. C. G. J., & Span, P. N. (2013). Hypoxia stimulates migration of breast cancer cells via the PERK/ATF4/LAMP3-arm of the unfolded protein response. Breast Cancer Res., 15, R2(13pp).
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• 7. Mees, G., Dierckx, R., Vangestel, Ch., & Van de Wiele,Ch. (2009). Molecular imaging of hypoxia with radiolabelled agents. Eur. J. Nucl. Med. Mol. Imaging, 36, 1675–1680.
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• 12. Arvold, N. D., Heidari, P., Kunawudhi, A., Sequist, L. V., & Mahmood, U. (2015). Tumor hypoxia response after targeted therapy in EGFR-mutant non-small cell lung cancer. Technol. Cancer Res. Treat., 15(2), 234–242.
• 13. Bruehlmeier, M., Kaser-Hotz, B., Achermann, R., Rohrer Bley, C., Wergin, M., Schubiger, P. A., & Ametamey, S. M. (2005). Measurement of tumor hypoxia in spontaneous canine sarcomas. Vet. Radiol. Ultrasoun., 46(4), 348–354.
• 14. Kilian, K., Chabecki, B., Kiec, J., Kunka, A., Panas, B., Wójcik, M., & Pekal, A. (2014). Synthesis, quality control and determination of metallic impurities in 18F-fludeoxyglucose production process. Rep. Pract. Oncol. Radiother., 19, 22–31.
• 15. Anzellotti, A., Bailey, J., Ferguson, D., McFarland, A., Bochev, P., Andreev, G., Awasthi, V., & Brown--Proctor, C. (2015). Automated production and quality testing of [18F]labeled radiotracers using the BG75 system. J. Radioanal. Nucl. Chem., 305(2), 387–401.
• 16. Blom, E., & Koziorowski, J. (2014). Automated synthesis of [18F]FMISO on IBA Synthera®. J. Radioanal. Nucl. Chem., 299(1), 265–270.
• 17. Nandy, S. K., & Rajan, M. (2010). Fully automated radiosynthesis of [18F]Fluoromisonidazole with single neutral alumina column purifi cation: optimization of reaction parameters. J. Radioanal. Nucl. Chem., 286(1), 241–248.
• 18. Bowen, S. R., van der Kogel, A. J., Nordsmark, M., Bentzen, M. S., & Jeraj, R. (2011). Characterization of PET hypoxia tracer uptake and tissue oxygenation via electrochemical modeling. Nucl. Med. Biol., 38(6), 771–780.

Uwagi

PL

Opracowanie ze środków MNiSW w ramach umowy 812/P-DUN/2016 na działalność upowszechniającą naukę.