Utilization of specific primers in legume allergens based polymorphism screening

• Autorzy: Klongová Lucia , Kováčik Adam , Urbanová Lucia , Kyseľ Matúš , Ivanišová Eva , Žiarovská Jana

Identyfikatory

DOI

10.5604/01.3001.0015.5431

• Języki publikacji: EN

Abstrakty

EN

Different types of allergies became a part of life of many people around the world. The research activities connecting to allergens are actually not oriented only for protein and immunological interactions, but to the genomic and transcriptomic background of them, too. Analysis and description of genomic variability of allergens in plant food resources will help to manage the allergen based strategies in the future. Here, the bioinformatic approach was used to develop and validate the specific primers for genomic screening of polymorphism of profilins (Profilin Based Amplicon Polymorphism; PBAP) and vicilins (Vicilin Based Amplicon Polymorphism; VBAP) among the legumes. The alignment of existing public databases data for these allergens in the group of legumes was performed. Subsequently, specific primers were designed and their ability to generate polymorphic amplicons were tested for three legumes – bean, lentil and chickpeas. In all cases, amplicons were generated and polymorphism was detected in all three species for profilin as well as for vicilin.

Słowa kluczowe

EN

legume; profilins; vicilin; specific primer; polymorphism

PL

roślina strączkowa; profiliny; wicylina; starter specyficzny; polimorfizm

• Wydawca: Państwowa Wyższa Szkoła Zawodowa w Tarnowie
• Czasopismo: Science, Technology and Innovation
• Rocznik: 2021
• Tom: Vol. 13, no. 2
• Strony: 12--21
• Opis fizyczny: Bibliogr. 61 poz., tab., wykr.

Twórcy

autor

Klongová Lucia

• AgroBioTech Research Centre, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic

autor

Kováčik Adam

• Institute of Plant and Environmental Sciences, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic

autor

Urbanová Lucia

• AgroBioTech Research Centre, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic

• https://orcid.org/0000-0001-7434-2677

autor

Kyseľ Matúš

• AgroBioTech Research Centre, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic

• https://orcid.org/0000-0002-1679-391X

autor

Ivanišová Eva

• Institute of Food Sciences, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76, Nitra, Slovak Republic

• https://orcid.org/0000-0001-5193-2957

autor

Žiarovská Jana

• Institute of Plant and Environmental Sciences, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic

• https://orcid.org/0000-0002-0005-9729

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