Morphological and Molecular Diversity of Benthic Cyanobacteria Communities Versus Environmental Conditions in Shallow, High Mountain Water Bodies in Eastern Pamir Mountains (Tajikistan)

• Autorzy: Jasser Iwona , Kostrzewska-Szlakowska Iwona , Kwiatowski Jan , Navruzshoev Dovutsho , Suska-Malawska Małgorzata , Khomutovska Nataliia

Identyfikatory

DOI

10.3161/15052249PJE2019.67.4.002

• Języki publikacji: EN

Abstrakty

EN

In mountain desert ecosystems, wetlands around saline and freshwater lakes allow various organisms to thrive and sometimes serve as the only source of drinking water for wild and domestic animals. We present results concerning diversity and structure of cyanobacterial inoculum from Eastern Pamir Mountains' benthic sediments, collected from small water bodies with contrasting salinity, temperature and other chemical parameters. We used morphological identification and molecular NGS techniques based on the amplification of the V3-V4 hypervariable region of 16S rRNA gene. Only a few cyanobacterial taxa have been identified in the preserved samples, while 27 taxa were successfully isolated and identified from the benthic sediments. Metagenomic analysis revealed that the cyanobacterial contribution to benthic bacterial communities was low. Representatives of the order Nostocales dominated in the samples, followed by Synechococcales, while contributions of Oscillatoriales and Chroococcales was much lower. The correlation matrix for the amplicon-based composition of samples clustered together samples of similar salinity and temperature. However, in hierarchical clustering of taxonomic structure of samples, communities with similar structures were not grouped by salinity or temperature. These results suggest that salinity and to some extent temperature, influence the composition of the inoculum, although the structure of the cyanobacterial communities is further shaped by other factors. Our study also demonstrated that the benthic inoculum for cyanobacterial communities contained potentially toxic taxa characteristic of both benthic and planktonic communities.

Słowa kluczowe

EN

16S rRNA amplicon; arid mountain environment; benthic communities; cyanobacterial diversity; NGS; salinity

PL

amplikon rRNA 16S; suche środowisko górskie; społeczności bentosowe; sinice; NGS; zasolenie

• Wydawca: Museum and Institute of Zoology, Polish Academy of Sciences
• Czasopismo: Polish Journal of Ecology
• Rocznik: 2019
• Tom: Vol. 67, nr 4
• Strony: 286--304
• Opis fizyczny: Bibliogr. 50 poz., rys., tab., wykr.

Twórcy

autor

Jasser Iwona

• University of Warsaw, Department of Plant Ecology and Environmental Conservation, Faculty of Biology, Warsaw, Poland

autor

Kostrzewska-Szlakowska Iwona

• University of Warsaw, Faculty of Biology, Miecznikowa 1, 02-089, Warsaw, Poland

autor

Kwiatowski Jan

• University of Warsaw, Faculty of Biology, Miecznikowa 1, 02-089, Warsaw, Poland
• Department of Ecology and Evolutionary Biology, University of California, Irvine, CA 92697, USA

autor

Navruzshoev Dovutsho

• Kh.Yu. Yusufbekov Pamir Biological Institute of the Academy of Sciences of the Republic of Tajikistan

autor

Suska-Malawska Małgorzata

• University of Warsaw, Department of Plant Ecology and Environmental Conservation, Faculty of Biology, Warsaw, Poland

autor

Khomutovska Nataliia

• University of Warsaw, Department of Plant Ecology and Environmental Conservation, Faculty of Biology, Warsaw, Poland

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Uwagi

Opracowanie rekordu ze środków MNiSW, umowa Nr 461252 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2020).