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Wybrane pełne teksty z tego czasopisma
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Warianty tytułu
Języki publikacji
Abstrakty
Dispersive liquid-liquid microextraction (DLLME) and high performance liquid chromatography – UV detection was presented for extraction and determination of nandrolone and testosterone in human urines. Chloroform at microliter volume level and acetonitrile were used as extraction and dispersive solvents, respectively. The main advantages of method are high speed, high enrichment factor, high recovery, good repeatability and extraction solvent volume at µL level. The influence of several variables (e.g. type and volume of disperser and extraction solvents, ionic strength, etc.) on the performance of the sample preparation step was carefully evaluated. Under the optimum conditions, the calibration graphs were linear in the range of 5–500 μg L⁻¹ with detection limit of 2.5 μg L⁻¹ for both of them. The relative standard deviation (R.S.D.s) for five replicate measurements of nandrolone and testosterone were 9.4% and 8.8%, respectively. The relative recoveries of nandrolone and testosterone in urine sample at spiking level of 25.0 μg L⁻¹ are ranged between 86.4% and 98%. DLLME combined with HPLC-UV is a fast, simple and efficient method for the determination of nandrolone and testosterone in human urines.
Czasopismo
Rocznik
Tom
Strony
28--34
Opis fizyczny
Bibliogr. 28 poz., tab., wykr.
Twórcy
autor
- Nuclear Fuel Cycle Research School, Nuclear Science and Technology Research Institute, Tehran, Iran
Bibliografia
- 1. Engel, L. L.; Alexander, J.; Wheeler, M. J. Biol. Chem. 1958, 231, 159.
- 2. Masse, R.; Laliberte, C.; Tremblay, L.; Dugal, R. Biomed. Mass Spectrom. 1985, 12, 115.
- 3. Pal, S. B. Clin. Chim. Acta 1971, 33, 215.
- 4. Venturelli, E.; Cavalleri, A.; Secreto, G. J. Chromatogr. B. 1995, 671, 363.
- 5. Shimada, K.; Mitamura, K.; Higashi, T. J. Chromatogr. A. 2001, 935, 141.
- 6. Choi, M. H.; Kim, J. K.; Chung, B. C. Analyst 1999, 124, 675.
- 7. Leinonen, A.; Kuuranne, T.; Kostiainen, R. J. Mass Spectrom. 2002, 37, 693.
- 8. Catlin, D. H.; Kammerer, R. C.; Hatton, C. K.; Sekera, M. H.; Merdink, J. L. Clin. Chem. 1987, 33, 319.
- 9. Gonzalo-Lumbreras, R.; Pimentel-Trapero, D.; Izquierdo-Hornillos, R. J. Chromatogr. Sci. 2003, 41, 261.
- 10. Li, L.; Liu, F.; Kong, X. X.; Su, S.; Li, K. A. Anal. Chim. Acta 2002, 452, 321.
- 11. He, C. Y.; Li, S. H.; Liu, H. W.; Li, K. A.; Liu, F. J. Chromatogr. A. 2005, 1082, 143.
- 12. Rezaee, M.; Assadi, Y.; Milani Hosseini, M. R.; Aghaee, E.; Ahmadi, F.; Berijani, S. J. Chromatogr. A. 2006, 1116, 1.
- 13. Berijani, S.; Assadi, Y.; Anbia, M.; Milani Hosseini, M. R.; Aghaee, E. J. Chromatogr. A. 2006, 1123, 1.
- 14. Rezaee, M.; Yamini, Y.; Faraji, M. J. Chromatogr. A. 2010, 1217, 2342.
- 15. Rezaee, M.; Yamini, Y.; Shariati, S.; Esrafili, A.; Shamsipur, M. J. Chromatogr. A. 2009, 1216, 1511.
- 16. Fattahi, N.; Assadi, Y.; Milani Hosseini, M. R. J. Chromatogr. A. 2007, 1157, 23.
- 17. Bidari, A.; Zeini jahromi, E.; Assadi, Y.; Milani hosseini, M. R.; Jamali, M. R. Microchim. J. 2007, 87, 6.
- 18. Zeini jahromi, E.; Bidari, A.; Assadi, Y.; Milani hosseini, M. R.; Jamali, M. R. Anal. Chim. Acta 2007, 585, 305.
- 19. Jiang, H.; Qin, Y.; Hu, B. Talanta 2008, 74, 1160.
- 20. Ahmadi-Jouibari, T.; Fattahi, N.; Shamsipur, M.; Pirsaheb, M. J. Pharm. Biomed. Anal. 2013, 85, 14.
- 21. Szarka, A.; Turkova, D.; Hrouzkova, S. J. Chromatogr. A. 2018, 1570, 126.
- 22. Cortada, C.; Vidal, L.; Pastor, R.; Santiago, N.; Canals, A. Anal. Chim. Acta 2009, 649, 218.
- 23. Lopez, M. G.; Rodriguez, I.; Cela, R. J. Chromatogr. A. 2007, 1166, 9.
- 24. Nagaraju, D.; Huang, S. D. J. Chromatogr. A. 2007, 1161, 89.
- 25. Farina, L.; Boido, E.; Carrau, F.; Dellacassa, E. J. Chromatogr. A. 2007, 1157, 46.
- 26. Farajzadeh, M. A.; Bahram, M.; Jonsson, J. A. Anal. Chim. Acta 2007, 591, 69.
- 27. Olędzka, I.; Kowalski, P.; Plenis, A.; Bączek, T. Electrophoresis 2017, 38, 1632.
- 28. Dmitrieva, E.; Temerdashev, A.; Azaryan, A.; Gashimova, E. J. Chromarogr. B 2020, 1159, 122390.
Uwagi
Opracowanie rekordu ze środków MEiN, umowa nr SONP/SP/546092/2022 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2022-2023).
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-638617c0-8083-4d37-94c8-ea7d11ed65a1