Covalent immobilization of laccase on Fe3O4-graphene oxide nanocomposite for biodegradation of phenolic compounds

• Autorzy: Rouhani Shamila , Azizi Shohreh , Maaza Malik , Mamba Bhekie , Msagati Titus A. M.

Identyfikatory

DOI

10.37190/epe210107

• Języki publikacji: EN

Abstrakty

EN

Laccase from Trametes Versicolor (E.C. 1.10.3.2) was immobilized on the Fe₃O₄–graphene hybrid nanocomposite through the covalent attachment method (Lac/Fe₃O₄/GO). The effect of immobilization conditions on the activity and recovered activities such as contact time, the concentration of glutaraldehyde and enzyme was evaluated. The recovered activity of the immobilized laccase on the Fe₃O4–graphene oxide nanocomposite was ca. 86%. Immobilized laccase unlike free laccase retained the activity and exhibited higher resistance to temperature and pH changes and also improved storage and thermal stability. Approximately 70% of relative activity for immobilized laccase was remained after being incubated for 2 h at 55 °C, but free laccase only remained 48%. Immobilized laccase retained 88% of initial activity after storage for 20 days, however, the free laccase only 32%. Finally, Lac/Fe₃O₄/GO capability was evaluated by the oxidation of phenol, p-chlorophenol, and 2,4-dichlorophenol. Lac/Fe3O4/GO was characterized by SEM, EDX, FT-IR, and AGFM.

Słowa kluczowe

EN

phenol; adsorption; biodegradation; Fe3O4; Trametes versicolor; laccase

PL

fenol; adsorbcja; biodegradacja; Fe3O4; lakaza; Trametes versicolor

• Wydawca: Oficyna Wydawnicza Politechniki Wrocławskiej
• Czasopismo: Environment Protection Engineering
• Rocznik: 2021
• Tom: Vol. 47, nr 1
• Strony: 85--99
• Opis fizyczny: Bibliogr. 25 poz., rys., tab.

Twórcy

autor

Rouhani Shamila

• Institute for Nanotechnology and Water Sustainability (iNanoWS) Unit, College of Science, Engineering and Technology, University of South Africa, Johannesburg, 1709, South Africa

autor

Azizi Shohreh

• UNESCO-UNISA Africa Chair in Nanoscience and Nanotechnology College of Graduates Studies, University of South Africa, Muckleneuk Ridge, Pretoria, South Africa, 392
• Nanosciences African Network (NANOAFNET), iThemba LABS-National Research Foundation, 1 Old Faure Road, Somerset West 7129, PO Box 722, Somerset West, Western Cape, South Africa

autor

Maaza Malik

• UNESCO-UNISA Africa Chair in Nanoscience and Nanotechnology College of Graduates Studies, University of South Africa, Muckleneuk Ridge, Pretoria, South Africa, 392
• Nanosciences African Network (NANOAFNET), iThemba LABS-National Research Foundation, 1 Old Faure Road, Somerset West 7129, PO Box 722, Somerset West, Western Cape, South Africa

autor

Mamba Bhekie

• State Key Laboratory of Separation Membranes and Membrane Process, National Center for International Joint Research on Membrane Science and Technology, Tianjin 300387, China
• Institute for Nanotechnology and Water Sustainability (iNanoWS) Unit, College of Science, Engineering and Technology, University of South Africa, Johannesburg, 1709, South Africa

autor

Msagati Titus A. M.

• Institute for Nanotechnology and Water Sustainability (iNanoWS) Unit, College of Science, Engineering and Technology, University of South Africa, Johannesburg, 1709, South Africa
• School of Life Sciences and Bio-Engineering, The Nelson Mandela African Institution of Science and Technology, Tengeru 447, Arusha, Tanzania

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Uwagi

Opracowanie rekordu ze środków MNiSW, umowa Nr 461252 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2021).