Modification of PLGA microspheres’ microstructure for application as cell carriers in modular tissue engineering

• Autorzy: Mielan B. , Krok-Borkowicz M. , Pielichowska K. , Pamuła E.
• Języki publikacji: EN

Abstrakty

EN

Microspheres (MS) made of resorbable polymer have been proposed as a cell growth support. They may be assembled to form cell constructs or be suspended in hydrogels allowing injection into injury location. High relative surface area of MS provides more efficient cell culture environment than traditional culture on flat substrates (multiwell plates, Petri dishes). In addition, MS structure, topography and surface chemistry can be modified to promote cell adhesion and proliferation. The aim of this study was to obtain resorbable poly(L-lactide-co-glycolide) (PLGA) MS and to modify their properties by changing manufacturing conditions of the oil-in-water emulsification to better control structural and microstructural properties of MS and their biological performance. To this end, water phase was modified by addition of NaCl to change ionic strength, while oil phase by addition of polyethylene glycol (PEG). Microstructural and thermal properties were assessed. Cytocompatibility tests and cell cultures with MG-63 cells were conducted to verify potential relevance of MS as cell carriers. The results showed that it is possible to obtain cytocompatible MS by oil-in-water emulsification method and to control diameter, porosity and crystallinity of MS with the use of additives to oil and/or water phases without negative changes in MS cytocompatibility. The results prove that modification of both phases make it possible to produce MS with desired/controllable properties like surface topography, porosity and crystallinity.

Słowa kluczowe

EN

regenerative medicine; cell cultures; bottom-up; PLGA; emulsification

• Wydawca: Polish Society for Biomaterials in Cracow
• Czasopismo: Engineering of Biomaterials
• Rocznik: 2017
• Tom: Vol. 20, no. 140
• Strony: 7--11
• Opis fizyczny: Bibliogr. 13 poz., tab., wykr., zdj.

Twórcy

autor

Mielan B.

• AGH University of Science and Technology, Faculty of Materials Science and Ceramics, Department of Biomaterials and Composites, al. Mickiewicza 30, 30-059 Kraków, Poland

autor

Krok-Borkowicz M.

• AGH University of Science and Technology, Faculty of Materials Science and Ceramics, Department of Biomaterials and Composites, al. Mickiewicza 30, 30-059 Kraków, Poland

autor

Pielichowska K.

• AGH University of Science and Technology, Faculty of Materials Science and Ceramics, Department of Biomaterials and Composites, al. Mickiewicza 30, 30-059 Kraków, Poland

autor

Pamuła E.

• AGH University of Science and Technology, Faculty of Materials Science and Ceramics, Department of Biomaterials and Composites, al. Mickiewicza 30, 30-059 Kraków, Poland

Bibliografia

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• [2] Nichol J.W., Khademhosseini A.: Modular tissue engineering : engineering biological tissues from the bottom up. Soft Matter: 5 (2007) 1312-1319.
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• [5] Hossain K.M.Z., Patel U., Ahmed I.: Development of microspheres for biomedical applications : a review. Prog Biomater: 4 (2015) 1-19.
• [6] Gabler F., Frauenschuh S., Ringe J., Brochhausen C., Götz P., Kirkpatrick C.J., Sittinger M., Schubert H., Zehbe R.: Emulsion- -based synthesis of PLGA-microspheres for the in vitro expansion of porcine chondrocytes. Biomolecular Engineering: 24 (2007) 515-520.
• [7] Schon B.S., Hooper G.J., Woodfield T.B.F.: Modular Tissue Assembly Strategies for Biofabrication of Engineered Cartilage 45 (2017) 100-114.
• [8] Lao L., Tan H., Wang Y., Gao C.: Chitosan modified poly (L-lactide) microspheres as cell microcarriers for cartilage tissue engineering. Colloids and Surfaces B: Biointerfaces 66 (2008) 218-225.
• [9] Hu L., Huang M, Wang J., Zhong Y., Luo Y.: Preparation of magnetic poly(lactic-co-glycolic acid) microspheres with a controllable particle size based on a composite emulsion and their release properties for curcumin loading. Journal of Applied Polymer Science 133 (2016) 1-8.
• [10] Bardouille C., Lehmann J., Heimann P., Jockusch H.: Growth and differentiation of permanent and secondary mouse myogenic cell lines on microcarriers. Appl Microbiol Biotechnol 55 (2001) 556-562.
• [11] Jeong Y.I., Song J.G., Kang S.S., Ryu H.H., Lee Y.H., Choi C., Shin B.A., Kim K.K., Ahn K.Y., Jung S.: Preparation of poly(DL- -lactide- co-glycolide) microspheres encapsulating all-trans retinoic acid. International Journal of Pharmaceutics 259 (2003) 79-91.
• [12] Liu X., Wang T., Chow L.C., Yang M., Mitchell J.W.: Effects of Inorganic Fillers on the Thermal and Mechanical Properties of Poly(lactic acid). International Journal of Polymer Science (2014) 1-8.
• [13] Krok M., Pamuła E.: Poly(L-lactide-co-glycolide) microporous membranes for medical applications produced with the use of polyethylene glycol as a pore former. J Appl Polym Sci 125 (2012) 187-199.

Uwagi

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2018).